Medina-Contreras J M L, Colado-Velázquez J, Gómez-Viquez N L, Mailloux-Salinas P, Pérez-Torres I, Aranda-Fraustro A, Carvajal K, Bravo G
Department of Pharmacobiology, Centro de Investigación y de Estudios Avanzados-Instituto Politécnico Nacional, Mexico City, Mexico.
Department of Pathology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
Int J Obes (Lond). 2017 May;41(5):750-758. doi: 10.1038/ijo.2017.33. Epub 2017 Feb 28.
Hypoestrogenic (HE) women are one of the most vulnerable groups for the development of obesity and its complications. Capsaicin and exercise have demonstrated to reduce body weight and to improve insulin sensitivity in different animal models, but it is unknown whether their combination could be useful in HE obese females.
We investigated whether topical capsaicin, exercise or their combination had better therapeutic effects in an obesity-hypoestrogenism model. Ovariectomized Wistar rats were given a 30% sucrose solution (HE-Obese (HEOb)) or purified water (HE) during 28 weeks ad libitum; four experimental groups per each condition. After shaving the abdominal skin, cold cream vehicle was applied to the Sedentary groups (Sed) and capsaicin cream 0.075% (0.6 mg kg per day) to the Capsaicin groups (Cap). Exercise (Ex) groups ran on a treadmill every day for 20 min at speeds from 9 to 18 m per min increased every 10 days; combination groups (Cap+Ex) were given topical capsaicin 90 min before exercise. The treatments were performed for 6 weeks, and caloric intake and body weight were monitored. At the end of the experimental protocol, glucose tolerance tests were performed, the animals were killed by decapitation; blood and organs were obtained to perform oxidative profile, histology, biochemical analyses and Western blot.
In HEOb rats, the combined therapy reduced caloric intake, body weight and abdominal fat in a higher proportion than the individual treatments; it also decreased insulin resistance (IR), oxidative stress and pancreatic islet size. It was the only treatment that significantly increased p-AMPK levels in the soleus muscle. In HE rats, topical capsaicin was the only treatment that reduced glucose intolerance and improved the oxidative profile in a higher proportion than the combined therapy or Ex alone.
Capsaicin per se or its combination with moderate exercise could be a useful therapy against complications linked to obesity-IR in HE females.
雌激素水平低(HE)的女性是肥胖及其并发症发生的最脆弱群体之一。辣椒素和运动已被证明可减轻不同动物模型的体重并改善胰岛素敏感性,但它们的联合应用对HE肥胖女性是否有用尚不清楚。
我们研究了局部应用辣椒素、运动或它们的联合应用在肥胖-雌激素缺乏模型中是否具有更好的治疗效果。将去卵巢的Wistar大鼠在28周内随意给予30%蔗糖溶液(HE-肥胖(HEOb))或纯净水(HE);每种情况分为四个实验组。剃去腹部皮肤后,给久坐组(Sed)涂抹冷霜载体,给辣椒素组(Cap)涂抹0.075%辣椒素乳膏(每天0.6毫克/千克)。运动(Ex)组每天在跑步机上以每分钟9至18米的速度跑步20分钟,速度每10天增加一次;联合组(Cap+Ex)在运动前90分钟局部应用辣椒素。治疗进行6周,并监测热量摄入和体重。在实验方案结束时,进行葡萄糖耐量试验,动物断头处死;获取血液和器官进行氧化状态、组织学、生化分析和蛋白质印迹分析。
在HEOb大鼠中,联合治疗比单独治疗更能降低热量摄入、体重和腹部脂肪;还降低了胰岛素抵抗(IR)、氧化应激和胰岛大小。它是唯一能显著提高比目鱼肌中p-AMPK水平的治疗方法。在HE大鼠中,局部应用辣椒素是唯一能降低葡萄糖不耐受并比联合治疗或单独运动更能改善氧化状态的治疗方法。
辣椒素本身或其与适度运动的联合应用可能是治疗HE女性肥胖-IR相关并发症的有效疗法。