Nishio Zenta, Onoe Chihiro, Ito Miwako, Tabiki Tadashi, Nagasawa Koichi, Miura Hideho
Tokyo University of Agriculture, 1737, Funako, Atsugi, Kanagawa 243-0034, Japan; NARO Hokkaido Agricultural Research Center, Memuro Research Station, 9-4 Shinsei-minami, Memuro, Kasai, Hokkaido 082-0081, Japan.
Obihiro University of Agriculture and Veterinary Medicine , Nishi 2-11, Inada, Obihiro, Hokkaido 080-8555 , Japan.
Breed Sci. 2016 Dec;66(5):668-675. doi: 10.1270/jsbbs.16097. Epub 2016 Aug 3.
Fusarium head blight (FHB) is one of the most devastating diseases of wheat ( L.), and the development of cultivars with FHB resistance is the most effective way to control the disease. Yumechikara is a Japanese hard red winter wheat cultivar that shows moderate resistance to FHB with superior bread-making quality. To identify quantitative trait loci (QTLs) for FHB resistance in Yumechikara, we evaluated doubled haploid lines derived from a cross between Yumechikara and a moderate susceptible cultivar, Kitahonami, for FHB resistance in a 5-year field trial, and we analyzed polymorphic molecular markers between the parents. Our analysis of these markers identified two FHB-resistance QTLs, one from Yumechikara and one from Kitahonami. The QTL from Yumechikara, which explained 36.4% of the phenotypic variation, was mapped on the distal region of chromosome 1BS, which is closely linked to the low-molecular-weight glutenin subunit gene and the glume color gene . The other QTL (from Kitahonami) was mapped on chromosome 3BS, which explained 11.2% of the phenotypic variation. The close linkage between the FHB-resistance QTL on 1BS, and brings an additional value of simultaneous screening for both quality and FHB resistance using the glume color.
赤霉病是小麦最具毁灭性的病害之一，培育抗赤霉病品种是防治该病最有效的方法。“梦之力”是日本的一个硬红冬小麦品种，对赤霉病表现出中等抗性，且具有优良的制面包品质。为了鉴定“梦之力”中抗赤霉病的数量性状位点（QTL），我们在一项为期5年的田间试验中，评估了由“梦之力”与中度感病品种“北之海”杂交产生的双单倍体系的赤霉病抗性，并分析了亲本间的多态性分子标记。对这些标记的分析确定了两个抗赤霉病QTL，一个来自“梦之力”，另一个来自“北之海”。来自“梦之力”的QTL解释了36.4%的表型变异，定位在1BS染色体的远端区域，该区域与低分子量谷蛋白亚基基因和颖壳颜色基因紧密连锁。另一个QTL（来自“北之海”）定位在3BS染色体上，解释了11.2%的表型变异。1BS染色体上的抗赤霉病QTL与[具体基因名称1]和[具体基因名称2]之间的紧密连锁，使得利用颖壳颜色同时筛选品质和抗赤霉病具有额外的价值。
