Department for Agrobiotechnology Tulln, University of Natural Resources and Life Sciences Vienna, Tulln, Austria.
Theor Appl Genet. 2011 Jul;123(2):293-306. doi: 10.1007/s00122-011-1584-x. Epub 2011 Apr 11.
While many reports on genetic analysis of Fusarium head blight (FHB) resistance in bread wheat have been published during the past decade, only limited information is available on FHB resistance derived from wheat relatives. In this contribution, we report on the genetic analysis of FHB resistance derived from Triticum macha (Georgian spelt wheat). As the origin of T. macha is in the Caucasian region, it is supposed that its FHB resistance differs from other well-investigated resistance sources. To introduce valuable alleles from the landrace T. macha into a modern genetic background, we adopted an advanced backcross QTL mapping scheme. A backcross-derived recombinant-inbred line population of 321 BC(2)F(3) lines was developed from a cross of T. macha with the Austrian winter wheat cultivar Furore. The population was evaluated for Fusarium resistance in seven field experiments during four seasons using artificial inoculations. A total of 300 lines of the population were genetically fingerprinted using SSR and AFLP markers. The resulting linkage map covered 33 linkage groups with 560 markers. Five novel FHB-resistance QTL, all descending from T. macha, were found on four chromosomes (2A, 2B, 5A, 5B). Several QTL for morphological and developmental traits were mapped in the same population, which partly overlapped with FHB-resistance QTL. Only the 2BL FHB-resistance QTL co-located with a plant height QTL. The largest-effect FHB-resistance QTL in this population mapped at the spelt-type locus on chromosome 5A and was associated with the wild-type allele q, but it is unclear whether q has a pleiotropic effect on FHB resistance or is closely linked to a nearby resistance QTL.
虽然在过去十年中已经发表了许多关于小麦赤霉病（FHB）抗性的遗传分析报告，但关于小麦近缘种来源的 FHB 抗性的信息却很有限。在本研究中，我们报告了源于粗山羊草（格鲁吉亚斯佩尔特小麦）的 FHB 抗性的遗传分析。由于粗山羊草的起源地在高加索地区，因此推测其 FHB 抗性与其他经过深入研究的抗性来源不同。为了将来自地方品种粗山羊草的有价值的等位基因引入现代遗传背景，我们采用了先进的回交 QTL 作图方案。从粗山羊草与奥地利冬小麦品种 Furore 的杂交后代中，我们开发了一个 321 个回交衍生重组自交系（BC(2)F(3)）群体，用于在四个季节的七个田间试验中进行人工接种以评估对赤霉病的抗性。利用 SSR 和 AFLP 标记对群体中的 300 个系进行了遗传指纹分析。得到的连锁图谱覆盖了 33 个连锁群，共有 560 个标记。在四个染色体（2A、2B、5A、5B）上发现了 5 个源于粗山羊草的新型 FHB 抗性 QTL。在同一群体中还定位了一些与形态和发育性状相关的 QTL，其中一些与 FHB 抗性 QTL 部分重叠。只有 2BL 的 FHB 抗性 QTL 与株高 QTL 共定位。该群体中最大效应的 FHB 抗性 QTL 位于 5A 染色体上的斯佩尔特型位点，与野生型等位基因 q 相关，但不清楚 q 是否对 FHB 抗性具有多效性，还是与附近的抗性 QTL 紧密连锁。
