Terasawa Yohei, Ito Miwako, Tabiki Tadashi, Nagasawa Koichi, Hatta Koichi, Nishio Zenta
NARO Hokkaido Agricultural Research Center, Memuro Research Station , 9-4 Shinsei-minami, Memuro, Kasai, Hokkaido 082-0081 , Japan.
NARO Hokkaido Agricultural Research Center, Memuro Research Station, 9-4 Shinsei-minami, Memuro, Kasai, Hokkaido 082-0081, Japan; Tokyo University of Agriculture, 1737, Funako, Atsugi, Kanagawa 243-0034, Japan.
Breed Sci. 2016 Sep;66(4):471-480. doi: 10.1270/jsbbs.16026. Epub 2016 Jul 5.
A quantitative trait locus (QTL) controlling wheat grain protein content (GPC) and flour protein content (FPC) was identified using doubled haploid (DH) lines developed from a cross between the hard red winter wheat variety 'Yumechikara' with a high protein content used for bread making, and the soft red winter wheat 'Kitahonami' with a low protein content used for Japanese white salted noodles. A single major QTL, , was identified on the short arm of wheat chromosome 2 for both the GPC and FPC over 3 years of testing. was mapped on the flanking region of microsatellite marker . The DH lines grouped by the haplotype of the closest flanking microsatellite marker showed differences of 1.0% and 1.1% in mean GPC and FPC, respectively. Yield-component-related traits were not affected by the haplotype of , and major North American hard red winter wheat varieties showed the high-protein haplotype. Unlike derived from tetraploid wheat, has no negative effects on yield-component-related traits and should be useful for wheat breeding to increase GPC and FPC.
利用由高蛋白硬红冬小麦品种“梦之力”（用于制作面包）与低蛋白软红冬小麦“北之海”（用于制作日本白盐面）杂交培育的双单倍体（DH）系，鉴定出一个控制小麦籽粒蛋白含量（GPC）和面粉蛋白含量（FPC）的数量性状位点（QTL）。在3年的测试中，在小麦2号染色体短臂上鉴定出一个单一的主要QTL，该QTL同时控制GPC和FPC。该QTL被定位在微卫星标记的侧翼区域。根据最接近的侧翼微卫星标记的单倍型对DH系进行分组，结果显示，平均GPC和FPC分别存在1.0%和1.1%的差异。产量构成相关性状不受该QTL单倍型的影响，北美主要的硬红冬小麦品种表现出高蛋白单倍型。与源自四倍体小麦的该QTL不同，该QTL对产量构成相关性状没有负面影响，应该有助于小麦育种提高GPC和FPC。
