Dababat Abdelfattah A, Ferney Gomez-Becerra Hugo, Erginbas-Orakci Gul, Dreisigacker Susanne, Imren Mustafa, Toktay Halil, Elekcioglu Halil I, Mekete Tesfamariam, Nicol Julie M, Ansari Omid, Ogbonnaya Francis
International Maize and Wheat Improvement Center (CIMMYT) , P.K. 39 Emek, 06511 Ankara , Turkey.
International Maize and Wheat Improvement Center (CIMMYT) , Mexico.
Breed Sci. 2016 Dec;66(5):692-702. doi: 10.1270/jsbbs.15158. Epub 2016 Oct 25.
To identify loci linked to nematode resistance genes, a total of 126 of CIMMYT advanced spring wheat lines adapted to semi-arid conditions were screened for resistance to , , and , of which 107 lines were genotyped with 1,310 DArT. Association of DArT markers with nematode response was analyzed using the general linear model. Results showed that 11 markers were associated with resistance to (pathotype Ha21), 25 markers with resistance to , and 9 significant markers were identified to be linked with resistance to . In this work we confirmed that chromosome 4A (~90-105 cM) can be a source of resistance to as has been recently reported. Other significant markers were also identified on chromosomal regions where no resistant genes have been reported for both nematodes species. These novel QTL were mapped to chromosomes 5A, 6A, and 7A for ; on chromosomes 1A, 1B, 3A, 3B, 6B, 7AS, and 7D for ; and on chromosomes 1D, 2A, and 5B for and represent potentially new loci linked to resistance that may be useful for selecting parents and deploying resistance into elite germplasm adapted to regions where nematodes are causing problem.
为了鉴定与线虫抗性基因相关的位点，对总共126份适应半干旱条件的国际玉米小麦改良中心（CIMMYT）春小麦高级品系进行了对禾谷孢囊线虫（Heterodera avenae）、菲利普孢囊线虫（Heterodera filipjevi）和燕麦真滑刃线虫（Aphelenchus avenae）的抗性筛选，其中107个品系用1310个多样性微阵列技术（DArT）标记进行了基因分型。使用一般线性模型分析DArT标记与线虫反应的关联。结果表明，11个标记与对禾谷孢囊线虫（致病型Ha21）的抗性相关，25个标记与对菲利普孢囊线虫的抗性相关，9个显著标记被鉴定为与对燕麦真滑刃线虫的抗性相关。在这项研究中，我们证实了4A染色体（约90 - 105厘摩）可以作为对禾谷孢囊线虫抗性的来源，正如最近所报道的那样。在两个线虫物种均未报道有抗性基因的染色体区域也鉴定出了其他显著标记。这些新的数量性状位点（QTL）被定位到5A、6A和7A染色体上以抗菲利普孢囊线虫；在1A、1B、3A、3B、6B、7AS和7D染色体上以抗禾谷孢囊线虫；在1D、2A和5B染色体上以抗燕麦真滑刃线虫，它们代表了与抗性相关的潜在新位点，可能有助于选择亲本并将抗性导入适应线虫成灾地区的优良种质中。
