Plant Breeding Institute, Christian-Albrechts University, Kiel, Germany.
Sci Rep. 2023 Nov 10;13(1):19642. doi: 10.1038/s41598-023-46551-9.
The ability of a plant parasitic nematode to infect and reproduce within a host plant depends on its genotype and the environmental conditions before and during infection. We studied the culturing conditions of the root lesion nematode Pratylenchus neglectus to produce inoculum for plant infection tests. Nematodes were either cultivated on carrot calli for different periods or directly isolated from the roots of the host plants. After infection of wheat and barley plants in the greenhouse, nematodes were quantified by RT-qPCR and by visual counting of the nematodes. We observed drastically reduced infection rates after long-term (> 96 weeks) cultivation on carrot callus. In contrast, fresh isolates from cereal roots displayed much higher pathogenicity. We recommend using root lesion nematodes cultivated on carrot calli no longer than 48 weeks to guarantee uniform infection rates.
植物寄生线虫在宿主植物内感染和繁殖的能力取决于其基因型以及感染前后的环境条件。我们研究了根结线虫 Pratylenchus neglectus 的培养条件，以生产用于植物感染测试的接种物。线虫要么在胡萝卜愈伤组织上培养不同的时间，要么直接从宿主植物的根部分离。在温室中感染小麦和大麦植物后，通过 RT-qPCR 和线虫的目视计数来量化线虫。我们观察到，在胡萝卜愈伤组织上长期（>96 周）培养后，感染率急剧下降。相比之下，来自谷类植物根部的新鲜分离物表现出更高的致病性。我们建议使用在胡萝卜愈伤组织上培养不超过 48 周的根结线虫，以保证均匀的感染率。
