Qian Y M, Huang Y H
Zhongguo Yao Li Xue Bao. 1989 Jan;10(1):61-5.
The effects of tetrandrine (Tet) on platelet aggregation and thromboxane A2 (TXA2) generation were studied in rabbit platelet-rich plasma (PRP) prepared by centrifugation. The effects of Tet on calmodulin activity in platelet extracts were also investigated by measuring calmodulin-sensitive phosphodiesterase activity. ADP, collagen or arachidonic acid (AA)-induced platelet aggregation was inhibited by Tet in a dose-dependent manner. TXA2 generation in PRP treated by Tet was markedly decreased in collagen-induced group, but was not altered in AA-induced group, suggesting that the release of AA from platelet phospholipids stimulated by collagen was blocked by Tet. Further experiments showed that the effects of Tet were related to its inhibition of calmodulin-dependent phosphodiesterase activity. There was evidence that the effects originated from its anti-calmodulin properties instead of its direct action on phosphodiesterase.
通过离心制备兔富血小板血浆（PRP），研究汉防己甲素（Tet）对血小板聚集和血栓素A2（TXA2）生成的影响。还通过测量钙调蛋白敏感的磷酸二酯酶活性来研究Tet对血小板提取物中钙调蛋白活性的影响。Tet以剂量依赖性方式抑制ADP、胶原或花生四烯酸（AA）诱导的血小板聚集。Tet处理的PRP中，胶原诱导组的TXA2生成明显减少，但AA诱导组未改变，这表明Tet阻断了胶原刺激的血小板磷脂中AA的释放。进一步实验表明，Tet的作用与其对钙调蛋白依赖性磷酸二酯酶活性的抑制有关。有证据表明，这些作用源于其抗钙调蛋白特性，而非对磷酸二酯酶的直接作用。
