Krueger Eric, Chang A Nicole, Brown Dale, Eixenberger Josh, Brown Raquel, Rastegar Sepideh, Yocham Katie M, Cantley Kurtis D, Estrada David
Micron School of Materials Science and Engineering, Boise State University, 1910 University Dr., Boise, ID 83725, United States.
Biomolecular Research Center, Boise State University, 1910 University Dr., Boise, ID 83725, United States; Physics Department, Boise State University, 1910 University Dr., Boise, ID 83725, United States.
ACS Biomater Sci Eng. 2016 Aug 8;2(8):1234-1241. doi: 10.1021/acsbiomaterials.6b00139. Epub 2016 Jun 24.
This study demonstrates the growth and differentiation of C2C12 myoblasts into functional myotubes on 3-dimensional graphene foam bioscaffolds. Specifically, we establish both bare and laminin coated graphene foam as a biocompatible platform for muscle cells and identify that electrical coupling stimulates cell activity. Cell differentiation and functionality is determined by the expression of myotube heavy chain protein and Ca fluorescence, respectively. Further, our data show that the application of a pulsed electrical stimulus to the graphene foam initiates myotube contraction and subsequent localized substrate movement of over 100 micrometers. These findings will further the development of advanced 3-dimensional graphene platforms for therapeutic applications and tissue engineering.
本研究证明了C2C12成肌细胞在三维石墨烯泡沫生物支架上生长并分化为功能性肌管。具体而言，我们将裸露的和层粘连蛋白包被的石墨烯泡沫均确立为肌肉细胞的生物相容性平台，并确定电耦合刺激细胞活性。细胞分化和功能分别通过肌管重链蛋白的表达和钙荧光来确定。此外，我们的数据表明，对石墨烯泡沫施加脉冲电刺激会引发肌管收缩以及随后超过100微米的局部底物移动。这些发现将推动用于治疗应用和组织工程的先进三维石墨烯平台的发展。
