Borsa Baris Ata, Aldag Mehmet Ersoy, Yilmaz Meric, Dalar Zeynep Gungordu, Ozalp Veli Cengiz
Clin Lab. 2016 Sep 1;62(9):1671-1674. doi: 10.7754/Clin.Lab.2016.160120.
Since Brucellosis is difficult to diagnose based on clinical symptoms, the diagnosis mostly relies on the results of serological testing. ODAK Brucella Coombs Gel Test is a novel and rapid gel microcolumn agglutination test which is performed in microcolumns containing gel matrix and Coombs antibodies. In this study, we aimed to compare ODAK Brucella Coombs Gel Test with other commonly used serological tests.
150 blood samples of patients, preliminarily diagnosed as Brucellosis, were included in this study. Rose Bengal (RB), ODAK Brucella Coombs Gel Test (CGT), Brucellacapt (BCAP), and Standard Agglutination Test (SAT) were performed for all samples. Also, Coombs Agglutination Test (CAT) was performed for all SAT negative samples. 1/160 and above titers were accepted as positive result except RB which is a qualitative test.
100 (67%) out of 150 samples were found positive by RB. All of the 50 RB negative samples were also found negative by SAT and CAT test. However, 2 (4%) and 7 (14%) of them were positive by CGT and BCAP tests, respectively. Additionally, among 100 RB positive samples, only 68, 77, and 87 were positive by SAT+CAT combination, CGT, and BCAP tests, respectively.
Currently, CGT is the only rapid (< 1 hour) serological test in which Coombs antibodies are used. Our results showed that negative results of RB, as a screening test, are not reliable enough as compared to CGT. However, positive RBT results confirmed with SAT were almost always, in most of the cases with higher titers, positive with CGT and BCAP. On the other hand, even if SAT is found negative with RB positivity, samples still must be investigated with CAT, CGT or BCAP. Consequently, CGT may be used as a rapid screening test instead of RB and it furthermore has similar sensitivity with the other confirmation tests in which Coombs antibodies are used. Therefore, ODAK Brucella Coombs Gel Test seems to be a very useful diagnostic tool for Brucellosis.
由于布鲁氏菌病难以基于临床症状进行诊断，诊断大多依赖血清学检测结果。ODAK布鲁氏菌库姆斯凝胶试验是一种新型快速凝胶微柱凝集试验，在含有凝胶基质和库姆斯抗体的微柱中进行。在本研究中，我们旨在比较ODAK布鲁氏菌库姆斯凝胶试验与其他常用血清学检测方法。
本研究纳入了150例初步诊断为布鲁氏菌病患者的血样。对所有样本进行了虎红试验（RB）、ODAK布鲁氏菌库姆斯凝胶试验（CGT）、布鲁氏菌捕获试验（BCAP）和标准凝集试验（SAT）。此外，对所有SAT阴性样本进行了库姆斯凝集试验（CAT）。除定性试验RB外，滴度1/160及以上被视为阳性结果。
150个样本中有100个（67%）经RB检测为阳性。50个RB阴性样本经SAT和CAT检测也均为阴性。然而，其中分别有2个（4%）和7个（14%）样本经CGT和BCAP检测为阳性。此外，在100个RB阳性样本中，经SAT+CAT联合检测、CGT检测和BCAP检测分别有68个、77个和87个样本呈阳性。
目前，CGT是唯一一种使用库姆斯抗体的快速（<1小时）血清学检测方法。我们的结果表明，作为筛查试验，RB的阴性结果与CGT相比不够可靠。然而，经SAT确认的阳性RBT结果在大多数情况下，滴度较高时，几乎总是经CGT和BCAP检测为阳性。另一方面，即使RB呈阳性但SAT检测为阴性，样本仍必须用CAT、CGT或BCAP进行检测。因此，CGT可作为RB的快速筛查试验，并且它与其他使用库姆斯抗体的确认试验具有相似的敏感性。所以，ODAK布鲁氏菌库姆斯凝胶试验似乎是一种非常有用的布鲁氏菌病诊断工具。
