Takei Mami, Ito Shinsaku, Tanaka Keisuke, Ishige Taichiro, Suzuki Yoshihito
a Department of Bioresource Science , Ibaraki University , Inashiki , Japan.
b Faculty of Applied Bioscience, Department of Bioscience , Tokyo University of Agriculture , Setagaya, Tokyo , Japan.
Biosci Biotechnol Biochem. 2017 Jun;81(6):1069-1077. doi: 10.1080/09168451.2017.1285685. Epub 2017 Feb 6.
Insect galls are abnormal plant tissues induced by parasitic insect(s) for use as their habitat. In previous work, we suggested that gall tissues induced by the aphid Tetraneura nigriabdominalis on Japanese elm trees are less responsive than leaf tissues to jasmonic acid (JA), which is involved in the production of volatile organic compounds as a typical defensive reaction of plants against attack by insect pests. A comprehensive analysis of gene expression by RNA sequencing indicated that the number of JA responsive genes was markedly lower in gall tissues than in leaf tissues. This suggests that gall tissues are mostly defective in JA signaling, although JA signaling is not entirely compromised in gall tissue. Gene ontology analysis sheds light on some stress-related unigenes with higher expression levels in gall tissues, suggesting that host plants sense aphids as a biotic stress but are defective in the JA-mediated defense response in gall tissues.
虫瘿是由寄生昆虫诱导形成的异常植物组织，用作它们的栖息地。在之前的研究中，我们发现日本榆树上由黑腹四脉绵蚜诱导形成的虫瘿组织，相较于叶片组织，对茉莉酸（JA）的反应较弱。茉莉酸参与植物挥发性有机化合物的合成，是植物抵御害虫攻击的典型防御反应。通过RNA测序对基因表达进行的全面分析表明，虫瘿组织中茉莉酸响应基因的数量明显低于叶片组织。这表明虫瘿组织在茉莉酸信号传导方面大多存在缺陷，尽管茉莉酸信号传导在虫瘿组织中并未完全受损。基因本体分析揭示了一些在虫瘿组织中表达水平较高的与胁迫相关的单基因，这表明寄主植物将蚜虫视为一种生物胁迫，但在虫瘿组织中茉莉酸介导的防御反应存在缺陷。
