Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden.
Department of Crop Production Ecology, Swedish University of Agricultural Sciences, 75007 Uppsala, Sweden.
Int J Mol Sci. 2017 Dec 19;18(12):2765. doi: 10.3390/ijms18122765.
Aphids are pests on many crops and depend on plant phloem sap as their food source. In an attempt to find factors improving plant resistance against aphids, we studied the effects of overexpression and down-regulation of the lipoxygenase gene in barley ( L.) on the performance of two aphid species. A specialist, bird cherry-oat aphid ( L.) and a generalist, green peach aphid ( Sulzer) were studied. overexpressing lines showed up-regulation of some other jasmonic acid (JA)-regulated genes, and antisense lines showed down-regulation of such genes. Overexpression or suppression of did not affect aphid settling or the life span on the plants, but in short term fecundity tests, overexpressing plants supported lower aphid numbers and antisense plants higher aphid numbers. The amounts and composition of released volatile organic compounds did not differ between control and overexpressing lines. Up-regulation of genes was similar for both aphid species. The results suggest that plays a role in the activation of JA-mediated responses and indicates the involvement of in basic defense responses.
蚜虫是许多作物的害虫，以植物韧皮部汁液为食。为了寻找提高植物抗蚜虫能力的因素，我们研究了过表达和下调大麦（ L.）中脂氧合酶基因对两种蚜虫物种表现的影响。研究了一种专食性的鸟樱桃燕麦蚜虫（ L.）和一种多食性的绿桃蚜虫（ Sulzer）。过表达系表现出一些其他茉莉酸（JA）调节基因的上调，反义系表现出这些基因的下调。过表达或抑制 并没有影响蚜虫的定殖或在植物上的寿命，但在短期繁殖力测试中，过表达植物支持的蚜虫数量较少，而反义植物的蚜虫数量较多。释放的挥发性有机化合物的数量和组成在对照和过表达系之间没有差异。两种蚜虫的基因上调相似。结果表明， 在 JA 介导的反应的激活中起作用，并表明其参与了基本防御反应。
