Matheis S, Yemelin A, Scheps D, Andresen K, Jacob S, Thines E, Foster A J
Institut für Biotechnologie und Wirkstoff-Forschung gGmbH (IBWF), Erwin-Schrödinger-Str. 56, D-67663 Kaiserslautern, Germany.
Institut für Biotechnologie und Wirkstoff-Forschung gGmbH (IBWF), Erwin-Schrödinger-Str. 56, D-67663 Kaiserslautern, Germany.
Microbiol Res. 2017 Mar;196:106-117. doi: 10.1016/j.micres.2016.12.010. Epub 2016 Dec 30.
The Magnaporthe oryzae genes FLB3 and FLB4, orthologues of the Aspergillus nidulans regulators of conidiation FlbC and FlbD, were inactivated. These genes encode C2H2 zinc finger and Myb-like transcription factors, respectively, in A. nidulans. Analysis of the resultant mutants demonstrated that FLB4 is essential for spore formation and that strains lacking this gene are fluffy in their colony morphology due to an inability to complete conidiophore formation. Meanwhile, FLB3 is required for normal levels of aerial mycelium formation. We identified genes dependent on both transcription factors using microarray analysis. This analysis revealed that the transcription of several genes encoding proteins implicated in sporulation in Magnaporthe oryzae and other filamentous fungi are affected by FLB3 or FLB4 inactivation. Furthermore, the microarray analysis indicates that Flb3p may effectively reprogramme the cell metabolically by repressing transcription of genes encoding biosynthetic enzymes and inducing transcription of genes encoding catabolic enzymes. Additionally, qRT-PCR was employed and showed that FLB3 and FLB4 transcripts are enriched in synchronously sporulating cultures, as were the transcripts of other genes that are necessary for normal conidiation, consistent with a role for their gene products in this process.
稻瘟病菌（Magnaporthe oryzae）的基因FLB3和FLB4（构巢曲霉（Aspergillus nidulans）中分生孢子形成调节因子FlbC和FlbD的直系同源基因）被失活。在构巢曲霉中，这些基因分别编码C2H2锌指蛋白和Myb样转录因子。对所得突变体的分析表明，FLB4对孢子形成至关重要，缺乏该基因的菌株由于无法完成分生孢子梗的形成，其菌落形态呈蓬松状。同时，正常水平的气生菌丝体形成需要FLB3。我们使用微阵列分析鉴定了依赖于这两种转录因子的基因。该分析表明，稻瘟病菌和其他丝状真菌中几个编码与孢子形成相关蛋白质的基因转录受FLB3或FLB4失活的影响。此外，微阵列分析表明，Flb3p可能通过抑制编码生物合成酶的基因转录和诱导编码分解代谢酶的基因转录，有效地对细胞进行代谢重编程。此外，采用qRT-PCR表明，FLB3和FLB4转录本在同步产孢培养物中富集，正常分生孢子形成所需的其他基因的转录本也是如此，这与其基因产物在该过程中的作用一致。
