Mei Meng, Zhou Yu, Peng Wenfang, Yu Chan, Ma Lixin, Zhang Guimin, Yi Li
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China.
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China.
Microbiol Res. 2017 Mar;196:118-128. doi: 10.1016/j.micres.2016.12.002. Epub 2016 Dec 14.
Yeast surface display (YSD) system has been widely used in protein engineering since it was established 20 years ago. Combined with fluorescence-activated cell sorting (FACS) technology and directed evolution, YSD has been proven of its extraordinary effectiveness for molecular engineering of various target proteins, especially for antibodies. Recently, a few remarkable efforts were exploited to modify the original Aga1-Aga2 YSD for the non-antibody protein engineering with successful outcomes, expanding its application on oxidase, Class II major histocompatibility complex (MHC-II), protease, sortase, lipoic acid ligase etc. Here, the methodologies of these optimized Aga1-Aga2 YSD technologies were introduced, and the recent progress of non-antibody protein engineering using these methods was summarized.
酵母表面展示（YSD）系统自20年前建立以来，已在蛋白质工程中得到广泛应用。结合荧光激活细胞分选（FACS）技术和定向进化，YSD已被证明在各种靶蛋白的分子工程中具有非凡的有效性，尤其是对于抗体。最近，人们做出了一些显著努力来改进原始的Aga1-Aga2 YSD用于非抗体蛋白质工程，并取得了成功，扩大了其在氧化酶、II类主要组织相容性复合体（MHC-II）、蛋白酶、分选酶、硫辛酸连接酶等方面的应用。在此，介绍了这些优化的Aga1-Aga2 YSD技术的方法，并总结了使用这些方法进行非抗体蛋白质工程的最新进展。
