Aguilar-Diaz Hugo, Canizalez-Roman Adrian, Nepomuceno-Mejia Tomas, Gallardo-Vera Francisco, Hornelas-Orozco Yolanda, Nazmi Kamran, Bolscher Jan G M, Carrero Julio Cesar, Leon-Sicairos Claudia, Leon-Sicairos Nidia
a CIASaP, Facultad de Medicina, Universidad Autónoma de Sinaloa. Cedros y Sauces, Fracc. Fresnos Culiacán 80246, Sinaloa, México.
b Departamento de Investigación, Hospital de la Mujer, Boulevard Miguel Tamayo Espinoza de los Monteros S/N, Col. Desarrollo Urbano Tres Ríos, Culiacán 80020, Sinaloa, México.
Biochem Cell Biol. 2017 Feb;95(1):82-90. doi: 10.1139/bcb-2016-0079. Epub 2016 Nov 30.
Giardia intestinalis is the most common infectious protozoan parasite in children. Despite the effectiveness of some drugs, the disease remains a major worldwide problem. Consequently, the search for new treatments is important for disease eradication. Biological molecules with antimicrobial properties represent a promising alternative to combat pathogens. Bovine lactoferrin (bLF) is a key component of the innate host defense system, and its peptides have exhibited strong antimicrobial activity. Based on these properties, we evaluated the parasiticidal activity of these peptides on G. intestinalis. Trophozoites were incubated with different peptide concentrations for different periods of time, and the growth or viability was determined by carboxyfluorescein-succinimidyl-diacetate-ester (CFDA) and propidium iodide (PI) staining. Endocytosis of peptides was investigated by confocal microscopy, damage was analyzed by transmission and scanning electron microscopy, and the type of programmed cell death was analyzed by flow cytometry. Our results showed that the LF peptides had giardicidal activity. The LF peptides interacted with G. intestinalis and exposure to LF peptides correlated with an increase in the granularity and vacuolization of the cytoplasm. Additionally, the formation of pores, extensive membrane disruption, and programmed cell death was observed in trophozoites treated with LF peptides. Our results demonstrate that LF peptides exhibit potent in vitro antigiardial activity.
肠道贾第虫是儿童中最常见的传染性原生动物寄生虫。尽管某些药物有效，但该疾病仍然是一个全球性的主要问题。因此，寻找新的治疗方法对于根除该疾病很重要。具有抗菌特性的生物分子是对抗病原体的一种有前途的替代方法。牛乳铁蛋白（bLF）是宿主先天防御系统的关键组成部分，其肽已表现出强大的抗菌活性。基于这些特性，我们评估了这些肽对肠道贾第虫的杀寄生虫活性。将滋养体与不同浓度的肽在不同时间段孵育，并通过羧基荧光素-琥珀酰亚胺基-二乙酸酯（CFDA）和碘化丙啶（PI）染色来测定生长或活力。通过共聚焦显微镜研究肽的内吞作用，通过透射和扫描电子显微镜分析损伤，并通过流式细胞术分析程序性细胞死亡的类型。我们的结果表明，乳铁蛋白肽具有杀贾第虫活性。乳铁蛋白肽与肠道贾第虫相互作用，并且暴露于乳铁蛋白肽与细胞质的颗粒度和空泡化增加相关。此外，在用乳铁蛋白肽处理的滋养体中观察到孔的形成、广泛的膜破坏和程序性细胞死亡。我们的结果表明，乳铁蛋白肽在体外表现出强大的抗贾第虫活性。
