College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
Parasitol Res. 2012 Nov;111(5):1929-35. doi: 10.1007/s00436-012-3035-2. Epub 2012 Jul 20.
Pulsatilla chinensis is a medicinal root plant that has been used to treat a wide range of disease conditions. Our study determined the antiprotozoal activity of various P. chinensis extracts and fractions against Giardia intestinalis including their effects on parasite growth, cell viability, adherence, and morphology. Ethyl acetate extracts (IC50 = 257.081 μg/ml) were the most active to inhibit the growth of G. intestinalis followed by aqueous extract (PWE), saponins, and n-butanol extract. The PWE and ethyl acetate extract inhibited G. intestinalis trophozoites adherence after 3 h of incubation and killed almost 50 % of the parasite population in a time-dependent manner. Changes in morphology, presence of precipitates in the cytoplasm, dissolved cytoplasm with large vacuole, break of flagella and ventral disk, membrane blebs, and intracellular and nuclear clearance of the treated trophozoites were observed by scanning and transmission electron microscopy. We demonstrated that P. chinensis induced these changes in G. intestinalis morphology and consequently has potential therapeutic use against giardiasis.
白头翁是一种药用根植物，已被用于治疗多种疾病。我们的研究确定了各种白头翁提取物和馏分对肠道贾第虫的抗原虫活性，包括它们对寄生虫生长、细胞活力、粘附和形态的影响。乙酸乙酯提取物（IC50=257.081μg/ml）对抑制肠道贾第虫的生长最有效，其次是水提取物（PWE）、皂素和正丁醇提取物。PWE 和乙酸乙酯提取物在孵育 3 小时后抑制肠道贾第虫滋养体的粘附，并以时间依赖性方式杀死近 50%的寄生虫。扫描和透射电子显微镜观察到形态变化、细胞质中出现沉淀物、细胞质溶解形成大空泡、鞭毛和腹盘断裂、膜泡、胞内和核内清除处理后的滋养体。我们证明白头翁诱导肠道贾第虫发生这些形态变化，因此具有治疗贾第虫病的潜在用途。
