Tang B, Liu C, Zhang Q-M, Ni M
Department of Orthopedics, Tai'an City Central Hospital, Tai'an, Shandong, China.
Eur Rev Med Pharmacol Sci. 2017 Jan;21(2):246-251.
Increasing evidence has revealed that miRNAs play a key role in the development and progression of tumor and are being explored as a prognostic biomarker. The aim of this study was to investigate the clinical significance of miR-490-3p expression in osteosarcoma.
Quantitative real-time PCR (qRT-PCR) was used to evaluate miR-490-3p level in osteosarcoma tissues. We also analyzed the correlations between the expression level of miR-490-3p and the clinical characteristics in cases of osteosarcoma. Also, Kaplan-Meier curve and the log-rank test were conducted to detect the prognostic value of miR-490-3p. Finally, univariable and multivariable Cox regression analyses were used to evaluate independent prognostic factors.
MiR-490-3p expression level was significantly downregulated in osteosarcoma tissues in comparison with noncancerous bone tissues. Moreover, low miR-490-3p expression was significantly associated with distant metastasis, advanced clinical stage and poor overall survival and relapse-free survival. Univariate and multivariate analysis results indicated that miR-490-3p was an independent prognostic factor in osteosarcoma.
Our results showed that miR-490-3p plays critical roles in osteosarcoma progression and serves as a novel prognostic indicator and a potential therapeutic target for osteosarcoma.
越来越多的证据表明，微小RNA（miRNAs）在肿瘤的发生和发展中起关键作用，并且正被探索作为一种预后生物标志物。本研究的目的是探讨miR-490-3p表达在骨肉瘤中的临床意义。
采用定量实时聚合酶链反应（qRT-PCR）评估骨肉瘤组织中miR-490-3p的水平。我们还分析了miR-490-3p表达水平与骨肉瘤病例临床特征之间的相关性。此外，采用Kaplan-Meier曲线和对数秩检验来检测miR-490-3p的预后价值。最后，使用单变量和多变量Cox回归分析来评估独立预后因素。
与非癌性骨组织相比，骨肉瘤组织中miR-490-3p表达水平显著下调。此外，低miR-490-3p表达与远处转移、临床晚期以及总体生存率和无复发生存率差显著相关。单变量和多变量分析结果表明，miR-490-3p是骨肉瘤的一个独立预后因素。
我们的结果表明，miR-490-3p在骨肉瘤进展中起关键作用，可作为骨肉瘤的一种新型预后指标和潜在治疗靶点。
