Zhou C, Tan D-M, Chen L, Xu X-Y, Sun C-C, Zong L-J, Han S, Zhang Y-Z
Qilu Hospital of Shandong University, Jinan, Shandong, China.
Eur Rev Med Pharmacol Sci. 2017 Jan;21(2):219-226.
MicroRNAs (miRs) function as either oncogenes or tumor suppressors in the progression of various human cancers, including cervical cancer. This study aimed to explore the role of miR-212 in cervical cancer and the mechanisms underlying this role.
Quantitative real-time polymerase chain reaction (RT-PCR) and Western blot assays were used to determine the expression levels of miR-212 and TCF7L2 in the cervical cancer cells. Cell proliferation invasion was examined using BrdU assays and transwell, respectively. A bioinformatics analysis was used to predict targets, and a dual-luciferase reporter system was applied for validation.
In our study, we demonstrated that miR-212 expression was significantly downregulated in cervical cancer tissues and cell lines. Moreover, the increased expression of miR-212 suppressed cell proliferation and invasion of cervical cancer cell lines in vitro. On the contrary, the decreased expression of miR-212 promoted cell proliferation and invasion of cervical cancer cell lines. Finally, the results of Western blot showed that overexpression of miR-212 dramatically suppressed the protein expression of TCF7L2. The knockdown of miR-212 showed the contrary effect. Luciferase reporter assay identified TCF7L2 as a novel direct target of miR-212.
Our results revealed that miR-212 inhibited cervical cancer metastasis and progression by targeting TCF7L2 expression.
微小RNA（miR）在包括宫颈癌在内的多种人类癌症进展中发挥癌基因或肿瘤抑制因子的作用。本研究旨在探讨miR-212在宫颈癌中的作用及其潜在机制。
采用定量实时聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测宫颈癌细胞中miR-212和TCF7L2的表达水平。分别使用BrdU检测法和Transwell检测细胞增殖和侵袭情况。利用生物信息学分析预测靶点，并应用双荧光素酶报告系统进行验证。
在本研究中，我们证明miR-212在宫颈癌组织和细胞系中的表达显著下调。此外，miR-212表达增加可抑制宫颈癌细胞系在体外的增殖和侵袭。相反，miR-212表达降低则促进宫颈癌细胞系的增殖和侵袭。最后，蛋白质免疫印迹结果显示，miR-212过表达显著抑制TCF7L2的蛋白表达。miR-212敲低则显示相反的效果。荧光素酶报告基因检测确定TCF7L2是miR-212的一个新的直接靶点。
我们的结果表明，miR-212通过靶向TCF7L2表达抑制宫颈癌转移和进展。
