环状FAM13B通过吸附miR-212、上调E2F5表达并激活P53通路促进肝细胞癌的增殖。
CircFAM13B promotes the proliferation of hepatocellular carcinoma by sponging miR-212, upregulating E2F5 expression and activating the P53 pathway.
作者信息
Xie Ying, Hang Xiaofeng, Xu Wensheng, Gu Jing, Zhang Yuanjing, Wang Jianrong, Zhang Xiucui, Cao Xinghao, Zhan Junjie, Wang Junxue, Gan Jianhe
机构信息
Department of Infectious Disease, The First Affiliated Hospital of Soochow University, 188 Shizi street, Suzhou, 215000, China.
Department of Infectious Disease, Changzheng Hospital, Naval Medical University, 415 Fengyang street, Shanghai, 200003, China.
出版信息
Cancer Cell Int. 2021 Aug 4;21(1):410. doi: 10.1186/s12935-021-02120-6.
BACKGROUND
Most of the biological functions of circular RNAs (circRNAs) and the potential underlying mechanisms in hepatocellular carcinoma (HCC) have not yet been discovered.
METHODS
In this study, using circRNA expression data from HCC tumor tissues and adjacent tissues from the Gene Expression Omnibus database, we identified out differentially expressed circRNAs and verified them by qRT-PCT. Functional experiments were performed to evaluate the effects of circFAM13B in HCC in vitro and in vivo.
RESULTS
We found that circFAM13B was the most significantly differentially expressed circRNA in HCC tissue. Subsequently, in vitro and in vivo studies also demonstrated that circFAM13B promoted the proliferation of HCC. Further studies revealed that circFAM13B, a sponge of miR-212, is involved in the regulation of E2F5 gene expression by competitively binding to miR-212, inhibits the activation of the P53 signalling pathway, and promotes the proliferation of HCC cells.
CONCLUSIONS
Our findings revealed the mechanism underlying the regulatory role played by circFAM13B, miR-212 and E2F5 in HCC. This study provides a new theoretical basis and novel target for the clinical prevention and treatment of HCC.
背景
环状RNA（circRNAs）的大多数生物学功能以及肝细胞癌（HCC）潜在的机制尚未被发现。
方法
在本研究中，利用基因表达综合数据库中HCC肿瘤组织和邻近组织的circRNA表达数据，我们鉴定出差异表达的circRNAs，并通过qRT - PCT进行验证。进行功能实验以评估circFAM13B在体外和体内对HCC的影响。
结果
我们发现circFAM13B是HCC组织中差异表达最显著的circRNA。随后，体外和体内研究还表明circFAM13B促进HCC的增殖。进一步研究揭示，circFAM13B作为miR - 212的海绵，通过与miR - 212竞争性结合参与E2F5基因表达的调控，抑制P53信号通路的激活，并促进HCC细胞的增殖。
结论
我们的研究结果揭示了circFAM13B、miR - 212和E2F5在HCC中发挥调控作用的机制。本研究为HCC的临床防治提供了新的理论依据和新靶点。
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