i3S, Instituto de Investigação e Inovação em Saúde, ‡INEB, Instituto de Engenharia Biomédica , Rua Alfredo Allen, 208, 4200-135 Porto, Portugal.
Department of Polymer Science & Engineering, Zhejiang University , Hangzhou 310027, China.
ACS Appl Mater Interfaces. 2017 Mar 8;9(9):7979-7989. doi: 10.1021/acsami.6b16415. Epub 2017 Feb 22.
Infection and thrombus formation are still the biggest challenges for the success of blood contact medical devices. This work aims the development of an antimicrobial and hemocompatible biomaterial coating through which selective binding of albumin (passivant protein) from the bloodstream is promoted and, thus, adsorption of other proteins responsible for bacterial adhesion and thrombus formation can be prevented. Polyurethane (PU) films were coated with hyaluronic acid, an antifouling agent, that was previously modified with thiol groups (HA-SH), using polydopamine as the binding agent. Octadecyl acrylate (C18) was used to attract albumin since it resembles the circulating free fatty acids and albumin is a fatty acid transporter. Thiol-ene "click chemistry" was explored for C18 immobilization on HA-SH through a covalent bond between the thiol groups from the HA and the alkene groups from the C18 chains. Surfaces were prepared with different C18 concentrations (0, 5, 10, and 20%) and successful immobilization was demonstrated by scanning electron microscopy (SEM), water contact angle determinations, X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). The ability of surfaces to bind albumin selectively was determined by quartz crystal microbalance with dissipation (QCM-D). Albumin adsorption increased in response to the hydrophobic nature of the surfaces, which augmented with C18 saturation. HA-SH coating reduced albumin adsorption to PU. C18 immobilized onto HA-SH at 5% promoted selective binding of albumin, decreased Staphylococcus aureus adhesion and prevented platelet adhesion and activation to PU in the presence of human plasma. C18/HA-SH coating was established as an innovative and promising strategy to improve the antimicrobial properties and hemocompatibility of any blood contact medical device.
感染和血栓形成仍然是血液接触医疗器械成功的最大挑战。本工作旨在开发一种抗菌和血液相容性生物材料涂层，通过该涂层促进来自血流的白蛋白（钝化蛋白）的选择性结合，从而防止其他负责细菌黏附和血栓形成的蛋白质的吸附。使用聚多巴胺作为结合剂，将具有巯基（HA-SH）的抗污剂透明质酸涂覆在聚氨酯（PU）薄膜上。十八烷丙烯酸酯（C18）用于吸引白蛋白，因为它类似于循环游离脂肪酸，而白蛋白是脂肪酸的转运蛋白。探索了硫醇-烯“点击化学”将 C18 通过 HA 中的巯基与 C18 链中的烯基之间的共价键固定在 HA-SH 上。通过改变 C18 浓度（0、5、10 和 20%）制备了不同的表面，并通过扫描电子显微镜（SEM）、水接触角测定、X 射线光电子能谱（XPS）和傅里叶变换红外光谱（FTIR）证明了成功的固定化。通过石英晶体微天平（QCM-D）测定表面选择性结合白蛋白的能力。白蛋白吸附量随表面疏水性的增加而增加，当 C18 饱和时吸附量增加。HA-SH 涂层减少了对 PU 的白蛋白吸附。在 HA-SH 上固定 5%的 C18 促进了白蛋白的选择性结合，减少了金黄色葡萄球菌的黏附，并防止了在人血浆存在下血小板对 PU 的黏附和激活。C18/HA-SH 涂层的建立是一种创新且有前途的策略，可改善任何血液接触医疗器械的抗菌性能和血液相容性。
