Yuan Huihui, Xue Chenli, Zhu Jiaqian, Yang Zhaogang, Lan Minbo
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
Department of Radiation Oncology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Polymers (Basel). 2021 Mar 18;13(6):934. doi: 10.3390/polym13060934.
To reduce the possibility of bacterial infection and implant-related complications, surface modification on polyurethane (PU) film is an ideal solution to endow hydrophobic PU with antibacterial and antifouling properties. In this work, a variety of polyhexamethylene guanidine/ hyaluronic acid (PHMG/HA) multilayer films were self-assembled layer-by-layer on PU films using polyanions, carboxyl-activated HA, and polycations PHMG by controlling the concentration of these polyelectrolytes as well as the number of layers self-assembled. Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) spectra, water contact angle (WCA), and A Atomic force microscope (AFM) of PU and modified PU films were studied. Protein adsorption and bacterial adhesion as well as the cytotoxicity against L929 of the film on selected PU-(PHMG/HA)/5-5 were estimated. The results showed that PU-(PHMG/HA)/5-5 had the best hydrophilicity among all the prepared films, possessing the lowest level of protein adsorption. Meanwhile, this film showed efficient broad-spectrum antibacterial performance as well as significant resistance of bacterial adhesion of more than a 99.9% drop for the selected bacteria. Moreover, almost no influence on cell viability of L929 enhanced the biocompatibility of film. Therefore, the modified PU films with admirable protein absorption resistance, antimicrobial performance, and biocompatibility would have promising applications in biomedical aspect.
为降低细菌感染和植入相关并发症的可能性，对聚氨酯（PU）薄膜进行表面改性是赋予疏水PU抗菌和防污性能的理想解决方案。在本工作中，通过控制聚电解质的浓度以及自组装层数，使用聚阴离子、羧基活化的透明质酸（HA）和聚阳离子聚六亚甲基胍（PHMG）在PU薄膜上逐层自组装了多种聚六亚甲基胍/透明质酸（PHMG/HA）多层薄膜。研究了PU和改性PU薄膜的衰减全反射傅里叶变换红外光谱（ATR-FTIR）、水接触角（WCA）和原子力显微镜（AFM）。对选定的PU-(PHMG/HA)/5-5薄膜进行了蛋白质吸附、细菌粘附以及对L929细胞的细胞毒性评估。结果表明，PU-(PHMG/HA)/5-5在所有制备的薄膜中具有最佳的亲水性，蛋白质吸附水平最低。同时，该薄膜表现出高效的广谱抗菌性能以及对选定细菌的显著抗细菌粘附能力，细菌粘附下降超过99.9%。此外，对L929细胞活力几乎没有影响，增强了薄膜的生物相容性。因此，具有令人钦佩的抗蛋白质吸附、抗菌性能和生物相容性的改性PU薄膜在生物医学方面将具有广阔的应用前景。
