Smida Amani, Ncibi Saida, Taleb Jihen, Ben Saad Anouar, Ncib Sana, Zourgui Lazhar
Laboratory of Active Biomolecules Valorisation, Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisie; Research of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, University of Gafsa, 2112, Tunisie.
Research of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, University of Gafsa, 2112, Tunisie; Faculty of Science, Jazan University, Saudi Arabia.
Biomed Pharmacother. 2017 Apr;88:844-851. doi: 10.1016/j.biopha.2017.01.105. Epub 2017 Feb 4.
Opuntia ficus indica (family Cactaceae) is a typical Mediterranean plant, mainly used in food and traditional folk medicine. The present study was designed to evaluate the protective effect of Opuntia ficus indica extract against chlorpyrifos (CPF)-induced immunotoxicity in rats. The experimental animals consisted of four groups of Wistar rats (5-6 weeks old) of eight each: a control group, a group treated with CPF (10mg/kg), a group treated with Opuntia ficus indica extract (100mg/kg), and a group treated with cactus extract then treated with CPF. These components were daily administered by gavage for 30days. After treatment, immunotoxicity was estimated by a count of thymocytes, splenocytes, stem cells in the bone marrow, relative weights of thymus and spleen, DNA aspects, and oxidative stress status in these organs. Results showed that CPF could induce thymus atrophy, splenomegaly, and a decrease in the cell number in the bone marrow. It also increased the oxidative stress markers resulting in elevated levels of the lipid peroxidation with a concomitant decrease in the levels of enzymatic antioxidants (SOD, CAT, GPx) in both spleen and thymus, and also degradation of thymocyte and splenocyte DNA. Consistent histological changes were found in the spleen and thymus under CPF treatment. However, administration of Opuntia ficus indica extract was found to alleviate this CPF-induced damage.
仙人掌（仙人掌科）是一种典型的地中海植物，主要用于食品和传统民间医学。本研究旨在评估仙人掌提取物对毒死蜱（CPF）诱导的大鼠免疫毒性的保护作用。实验动物由四组Wistar大鼠（5 - 6周龄）组成，每组8只：对照组、用CPF（10mg/kg）处理的组、用仙人掌提取物（100mg/kg）处理的组以及先用仙人掌提取物处理然后用CPF处理的组。这些成分每天通过灌胃给药30天。处理后，通过计数胸腺细胞、脾细胞、骨髓干细胞、胸腺和脾脏的相对重量、DNA情况以及这些器官中的氧化应激状态来评估免疫毒性。结果表明，CPF可诱导胸腺萎缩、脾肿大以及骨髓细胞数量减少。它还增加了氧化应激标志物，导致脂质过氧化水平升高，同时脾脏和胸腺中酶促抗氧化剂（超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶）水平降低，以及胸腺细胞和脾细胞DNA降解。在CPF处理下，脾脏和胸腺中发现了一致的组织学变化。然而，发现给予仙人掌提取物可减轻这种CPF诱导的损伤。
