Petruk Ganna, Di Lorenzo Flaviana, Imbimbo Paola, Silipo Alba, Bonina Andrea, Rizza Luisa, Piccoli Renata, Monti Daria Maria, Lanzetta Rosa
Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte Sant'Angelo, via Cinthia 4, 80126 Naples, Italy.
Bionap srl, R&D Contrada Fureria, ZIO 950125 Piano Tavola, Belpasso, Italy.
Bioorg Med Chem Lett. 2017 Dec 15;27(24):5485-5489. doi: 10.1016/j.bmcl.2017.10.043. Epub 2017 Oct 20.
Opuntia ficus-indica L. is known for its beneficial effects on human health, but still little is known on cladodes as a potent source of antioxidants. Here, a direct, economic and safe method was set up to obtain water extracts from Opuntia ficus-indica cladodes rich in antioxidant compounds. When human keratinocytes were pre-treated with the extract before being exposed to UVA radiations, a clear protective effect against UVA-induced stress was evidenced, as indicated by the inhibition of stress-induced processes, such as free radicals production, lipid peroxidation and GSH depletion. Moreover, a clear protective effect against apoptosis in pre-treated irradiated cells was evidenced. We found that eucomic and piscidic acids were responsible for the anti-oxidative stress action of cladode extract. In conclusion, a bioactive, safe, low-cost and high value-added extract from Opuntia cladodes was obtained to be used for skin health/protection.
仙人掌（Opuntia ficus-indica L.）因其对人体健康有益而闻名，但对于其茎片作为强大抗氧化剂来源的了解仍然很少。在此，建立了一种直接、经济且安全的方法来从富含抗氧化化合物的仙人掌茎片中获取水提取物。当人角质形成细胞在暴露于紫外线A辐射之前用该提取物进行预处理时，对紫外线A诱导的应激有明显的保护作用，这表现为对应激诱导过程的抑制，如自由基产生、脂质过氧化和谷胱甘肽消耗。此外，在预处理的辐照细胞中对细胞凋亡有明显的保护作用。我们发现杜仲酸和鱼石脂酸是茎片提取物抗氧化应激作用的原因。总之，获得了一种具有生物活性、安全、低成本且高附加值的仙人掌茎片提取物，可用于皮肤健康/保护。
