Marvi -, Iqbal Javeid, Muhammad Shafi, Ahmad Mansoor
Department of Pharmacology, Faculty of Pharmacy, University of Balochistan, Quetta, Pakistan.
Department of Pharmacognosy, Faculty of Pharmacy, University of Balochistan, Quetta, Pakistan.
Pak J Pharm Sci. 2016 Nov;29(6 Suppl):2335-2341.
Present study was conducted on crude methanolic extract of stem and root of Taverniera glabra. In Pakistan T. glabra is found in the region of Balochistan only. T. glabra has numerous therapeutic uses in traditional medicine and it is also used for the pain relief. Current study was carried out to evaluate acute toxicity, analgesic and CNS depressant activity of the plant. Acute toxicity was carried out by oral administration of the T. glabra extract from 250 to 2000mg/kg oral dose. Analgesic activity was carried out by acetic acid induced writhing test and formalin test. Central Nervous System (CNS) depressant activity was carried out by exploratory activities (open field activity, cage crossing activity, rearing test) and forced swimming test. Oral administration of the methanolic extract of T. glabra was nontoxic at the dose of 1500mg/kg in the acute toxicity test. Exploratory behavior of mice treated with the methanolic extract of T. glabra showed sedative effects (P<0.05) in open field, cage crossing, traction and rearing test, particularly at the dose of 500mg as compared with standard drug Diazepam. In forced swimming test, mobility time was significantly (P<0.05) increased at 500mg/kg oral dose, and results were significant as compared with control. Methanolic extract of T. glabra produced significant (P<0.05) analgesic effects at the dose of 500mg/kg in the acetic acid induced writhing test and the formalin test. In conclusion, results show that the crude methanolic extract of T. glabra possess sedative as well as potent analgesic effects. Present pharmacological studies are the first ever studies conducted on the methanolic extract of T. glabra.
本研究以光果小苦荬茎和根的甲醇粗提物为对象展开。在巴基斯坦，光果小苦荬仅在俾路支省地区被发现。光果小苦荬在传统医学中有多种治疗用途，也被用于缓解疼痛。本研究旨在评估该植物的急性毒性、镇痛及中枢神经系统抑制活性。通过口服给予250至2000mg/kg口服剂量的光果小苦荬提取物进行急性毒性试验。通过醋酸诱导扭体试验和福尔马林试验进行镇痛活性研究。通过探索性活动（旷场活动、穿梭箱活动、竖毛试验）和强迫游泳试验进行中枢神经系统抑制活性研究。在急性毒性试验中，口服光果小苦荬甲醇提取物1500mg/kg剂量时无毒。用该提取物处理的小鼠在旷场、穿梭箱、竖毛和竖毛试验中的探索行为显示出镇静作用（P<0.05），尤其是在500mg剂量时，与标准药物地西泮相比。在强迫游泳试验中，口服500mg/kg剂量时活动时间显著增加（P<0.05），与对照组相比结果显著。光果小苦荬甲醇提取物在醋酸诱导扭体试验和福尔马林试验中500mg/kg剂量时产生显著（P<0.05）镇痛作用。总之，结果表明光果小苦荬甲醇粗提物具有镇静和强效镇痛作用。目前的药理学研究是首次对光果小苦荬甲醇提取物进行的研究。
