巴西里约热内卢产NDM-1肺炎克雷伯菌分离株的多克隆扩增
Multiclonal Expansion of Klebsiella pneumoniae Isolates Producing NDM-1 in Rio de Janeiro, Brazil.
作者信息
Aires Caio Augusto Martins, Pereira Polyana Silva, de Araujo Carlos Felipe Machado, Chagas Thiago Pavoni Gomes, Oliveira Jane Cleide Ribeiro, Buonora Sibelle Nogueira, Albano Rodolpho Mattos, Carvalho-Assef Ana Paula D'Alincourt, Asensi Marise Dutra
机构信息
Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
Laboratório Central de Saúde Pública Noel Nutels, Rio de Janeiro, Brazil.
出版信息
Antimicrob Agents Chemother. 2017 Mar 24;61(4). doi: 10.1128/AAC.01048-16. Print 2017 Apr.
Abstract
We characterized NDM-1-producing isolates from Rio de Janeiro, Brazil. PCR was applied for resistance and virulence determinants. The genetic context of was determined by S1 nuclease pulsed-field gel electrophoresis (PFGE) and hybridization. Genotyping was performed by PFGE and multilocus sequence typing (MLST). Most isolates carried multiple resistance genes and remained susceptible to amikacin, fosfomycin-trometamol, polymyxin B, and tigecycline. The spread of NDM-1-producing was not associated with clonal expansion and appears to be associated with Tn.
摘要
我们对来自巴西里约热内卢的产NDM-1菌株进行了特征分析。采用PCR检测耐药性和毒力决定因素。通过S1核酸酶脉冲场凝胶电泳（PFGE）和杂交确定blaNDM-1的基因背景。通过PFGE和多位点序列分型（MLST）进行基因分型。大多数菌株携带多个耐药基因，并且对阿米卡星、磷霉素氨丁三醇、多粘菌素B和替加环素仍敏感。产NDM-1菌株的传播与克隆扩增无关，似乎与转座子Tn有关。
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