Khalid Annum, Zhang Qingling, Yasir Muhammad, Li Feng
Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan, China.
Front Microbiol. 2017 Jan 23;8:43. doi: 10.3389/fmicb.2017.00043. eCollection 2017.
Small RNAs regulate a large set of gene expression in all plants and constitute a natural immunity against viruses. Small RNA based genetic engineering (SRGE) technology had been explored for crop protection against viruses for nearly 30 years. Viral resistance has been developed in diverse crops with SRGE technology and a few viral resistant crops have been approved for commercial release. In this review we summarized the efforts generating viral resistance with SRGE in different crops, analyzed the evolution of the technology, its efficacy in different crops for different viruses and its application status in different crops. The challenge and potential solution for application of SRGE in crop protection are also discussed.
小RNA调控所有植物中的大量基因表达，并构成对病毒的天然免疫。基于小RNA的基因工程（SRGE）技术已被探索用于作物抗病毒保护近30年。利用SRGE技术已在多种作物中培育出抗病毒性，一些抗病毒作物已获批用于商业种植。在本综述中，我们总结了利用SRGE在不同作物中产生抗病毒性的工作，分析了该技术的发展历程、其在不同作物中对不同病毒的有效性及其在不同作物中的应用现状。还讨论了SRGE在作物保护应用中的挑战和潜在解决方案。
