通过拉曼光谱研究偏端霉素-DNA和纺锤菌素-DNA复合物的构象特征
Conformational features of distamycin-DNA and netropsin-DNA complexes by Raman spectroscopy.
作者信息
Martin J C, Wartell R M, O'Shea D C
出版信息
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5483-7. doi: 10.1073/pnas.75.11.5483.
Abstract
The binding of distamycin and netropsin to duplex DNA has been studied by Raman spectroscopy. Several changes occur in the Raman spectra of these drugs upon binding DNA. These changes were analyzed by assigning specific motions to the observed Raman bands through the use of molecular subunits of the drugs and normal mode calculations. Our analysis indicates that pyrrole ring and peptide group vibrations are altered upon binding to DNA. The environments of the pyrrole ring methyl groups are not affected by the binding. These data provide physical evidence consistent with a binding model in which the methyl groups on the pyrroles project away from the DNA and the peptide N-H groups form hydrogen bonds with the DNA.
摘要
通过拉曼光谱研究了偏端霉素和纺锤菌素与双链DNA的结合。这些药物与DNA结合后，其拉曼光谱发生了若干变化。通过利用药物的分子亚基并进行简正模式计算，将特定运动赋予观察到的拉曼谱带，从而对这些变化进行了分析。我们的分析表明，吡咯环和肽基团的振动在与DNA结合后发生了改变。吡咯环甲基的环境不受结合的影响。这些数据提供了与一种结合模型相一致的物理证据，在该模型中，吡咯上的甲基远离DNA伸出，而肽的N-H基团与DNA形成氢键。
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本文引用的文献
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Conformation dependent binding of netropsin and distamycin to DNA and DNA model polymers.纺锤菌素和偏端霉素与DNA及DNA模型聚合物的构象依赖性结合
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Interaction of the oligopeptide antibiotics netropsin and distamycin A with nucleic acids.寡肽抗生素纺锤菌素和偏端霉素A与核酸的相互作用。
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Adenosine-thymidine cluster-specific elongation and stiffening of DNA induced by the oligopeptide antibiotic netropsin.寡肽抗生素纺锤菌素诱导的腺苷-胸腺嘧啶簇特异性DNA延伸与硬化
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