Baumeister W, Kübler O
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5525-8. doi: 10.1073/pnas.75.11.5525.
The paracrystalline outer membraneous layer (HPI layer) of Micrococcus radiodurans has been investigated by negative- and positive-staining electron microscopy and subsequent digital image processing. The subunit structure of the major HPI layer protein complex and the lipid-protein distribution in the plane of the membrane have been determined. The HPI layer was found to be highly asymmetric in a transmembrane direction, with the major protein complex only partly penetrating into a lipid-containing backing layer intimately associated with it.
通过负染色和正染色电子显微镜以及后续的数字图像处理，对耐辐射微球菌的准晶外膜层（HPI层）进行了研究。已经确定了主要HPI层蛋白复合物的亚基结构以及膜平面内的脂蛋白分布。发现HPI层在跨膜方向上高度不对称，主要蛋白复合物仅部分穿透与其紧密相关的含脂背衬层。
