Komatsu Toshiyuki, Sasaki Suguru, Manabe Yuki, Hirata Takashi, Sugawara Tatsuya
Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
PLoS One. 2017 Feb 7;12(2):e0171178. doi: 10.1371/journal.pone.0171178. eCollection 2017.
Astaxanthin, a carotenoid found mainly in seafood, has potential clinical applications due to its antioxidant activity. In this study, we evaluated the effect of dietary astaxanthin derived from Haematococcus pluvialis on skin photoaging in UVA-irradiated hairless mice by assessing various parameters of photoaging. After chronic ultraviolet A (UVA) exposure, a significant increase in transepidermal water loss (TEWL) and wrinkle formation in the dorsal skin caused by UVA was observed, and dietary astaxanthin significantly suppressed these photoaging features. We found that the mRNA expression of lympho-epithelial Kazal-type-related inhibitor, steroid sulfatase, and aquaporin 3 in the epidermis was significantly increased by UVA irradiation for 70 days, and dietary astaxanthin significantly suppressed these increases in mRNA expression to be comparable to control levels. In the dermis, the mRNA expression of matrix metalloprotease 13 was increased by UVA irradiation and significantly suppressed by dietary astaxanthin. In addition, HPLC-PDA analysis confirmed that dietary astaxanthin reached not only the dermis but also the epidermis. Our results indicate that dietary astaxanthin accumulates in the skin and appears to prevent the effects of UVA irradiation on filaggrin metabolism and desquamation in the epidermis and the extracellular matrix in the dermis.
虾青素是一种主要存在于海鲜中的类胡萝卜素，因其抗氧化活性而具有潜在的临床应用价值。在本研究中，我们通过评估光老化的各种参数，研究了雨生红球藻来源的膳食虾青素对UVA照射的无毛小鼠皮肤光老化的影响。长期暴露于紫外线A（UVA）后，观察到UVA导致背部皮肤的经表皮水分流失（TEWL）和皱纹形成显著增加，而膳食虾青素显著抑制了这些光老化特征。我们发现，UVA照射70天后，表皮中淋巴细胞上皮Kazal型相关抑制剂、类固醇硫酸酯酶和水通道蛋白3的mRNA表达显著增加，而膳食虾青素显著抑制了这些mRNA表达的增加，使其与对照水平相当。在真皮中，基质金属蛋白酶13的mRNA表达因UVA照射而增加，并被膳食虾青素显著抑制。此外，HPLC-PDA分析证实，膳食虾青素不仅能到达真皮，还能到达表皮。我们的结果表明，膳食虾青素在皮肤中积累，似乎可以预防UVA照射对表皮中丝聚蛋白代谢和脱屑以及真皮中细胞外基质的影响。
