Marshall H F, Shaffer G P, Conkerton E J
U.S. Department of Agriculture, Southern Regional Research Center, New Orleans, Louisiana 70179.
Anal Biochem. 1989 Aug 1;180(2):264-8. doi: 10.1016/0003-2697(89)90428-4.
A method which circumvents the lengthy procedural steps for sample preparation in the analysis of free amino acids (FAA) in the peanut is described. Sample preparation consists of extracting a full fat meal with a lithium citrate sample buffer for 15 min. The sample is centrifuged and filtered with two Millipore filters in series. The resulting extract is analyzed "as is" for FAA via a physiological amino acid procedure. The method significantly reduces sample preparation time and eliminates the use of hazardous chemicals. It is well adapted to the analysis of large numbers of samples or samples of limited quantity. It should be applicable to the analysis of FAA in other oilseeds.
本文描述了一种在花生游离氨基酸（FAA）分析中规避冗长样品制备程序步骤的方法。样品制备包括用柠檬酸锂样品缓冲液萃取全脂花生粕15分钟。样品经离心处理，然后通过两个串联的密理博过滤器过滤。所得提取物直接通过生理氨基酸分析程序进行FAA分析。该方法显著减少了样品制备时间，并避免了使用危险化学品。它非常适合分析大量样品或少量样品。该方法应适用于其他油籽中FAA的分析。
