Hugo Ayelén A, Rolny Ivanna S, Romanin David, Pérez Pablo F
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Universidad Nacional de La Plata (UNLP), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-CCT La Plata, La Plata, Argentina.
Cátedra de Microbiología, Facultad de Ciencias Exactas (FCE), UNLP, La Plata, Argentina.
World J Microbiol Biotechnol. 2017 Mar;33(3):48. doi: 10.1007/s11274-017-2219-4. Epub 2017 Feb 7.
Citrobacter rodentium is a specific murine enteropathogen which causes diarrheal disease characterized by colonic hyperplasia and intestinal inflammation. Recruitment of neutrophils and macrophages constitute a key step to control the infection. Since modulation of the activity of professional phagocytic cells could contribute to improve host´s defences against C. rodentium, we investigated the effect of Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) on the interaction between murine macrophages (RAW 264.7) and C. rodentium. Phagocytosis, surface molecules and inducible nitric oxide synthase (iNOs) expression were determined by flow cytometry. Reactive oxygen species (ROS) were assessed by fluorescence microscopy. The presence of lactobacilli increased phagocytosis of C. rodentium whereas C. rodentium had no effect on lactobacilli internalization. Survival of internalized C. rodentium diminished when strain CIDCA 133 was present. CD-86, MHCII, iNOs expression and nitrite production were increased when C. rodentium and lactobacilli were present even though strain CIDCA 133 alone had no effect. Strain CIDCA 133 led to a strong induction of ROS activity which was not modified by C. rodentium. Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) is able to increase the activation of murine macrophages infected with C. rodentium. The sole presence of lactobacilli is enough to modify some stimulation markers (e.g. ROS induction) whereas other markers require the presence of both bacteria; thus, indicating a synergistic effect.
鼠柠檬酸杆菌是一种特定的鼠肠道病原体，可引起以结肠增生和肠道炎症为特征的腹泻病。中性粒细胞和巨噬细胞的募集是控制感染的关键步骤。由于调节专业吞噬细胞的活性有助于改善宿主对鼠柠檬酸杆菌的防御能力，我们研究了德氏乳杆菌乳酸亚种（菌株CIDCA 133）对鼠巨噬细胞（RAW 264.7）与鼠柠檬酸杆菌之间相互作用的影响。通过流式细胞术测定吞噬作用、表面分子和诱导型一氧化氮合酶（iNOs）的表达。通过荧光显微镜评估活性氧（ROS）。乳酸杆菌的存在增加了鼠柠檬酸杆菌的吞噬作用，而鼠柠檬酸杆菌对乳酸杆菌的内化没有影响。当存在菌株CIDCA 133时，内化的鼠柠檬酸杆菌的存活率降低。当存在鼠柠檬酸杆菌和乳酸杆菌时，CD-86、MHCII、iNOs表达和亚硝酸盐产生增加，尽管单独的菌株CIDCA 133没有影响。菌株CIDCA 133导致ROS活性的强烈诱导，而鼠柠檬酸杆菌对此没有改变。德氏乳杆菌乳酸亚种（菌株CIDCA 133）能够增加感染鼠柠檬酸杆菌的鼠巨噬细胞的活化。仅乳酸杆菌的存在就足以改变一些刺激标志物（如ROS诱导），而其他标志物则需要两种细菌都存在；因此，表明存在协同作用。
