De Jesus Luís Cláudio Lima, Aburjaile Flávia Figueira, Sousa Thiago De Jesus, Felice Andrei Giacchetto, Soares Siomar De Castro, Alcantara Luiz Carlos Junior, Azevedo Vasco Ariston De Carvalho
Department of Genetics, Ecology and Evolution, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Department of Preventive Veterinary Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Front Bioinform. 2022 Jun 6;2:912795. doi: 10.3389/fbinf.2022.912795. eCollection 2022.
Probiotics are health-beneficial microorganisms with mainly immunomodulatory and anti-inflammatory properties. species is a common bacteria used in the dairy industry, and their benefits to hosting health have been reported. This study analyzed the core genome of nine strains of species with documented probiotic properties, focusing on genes related to their host health benefits. For this, a combined methodology including several software and databases (BPGA, SPAAN, BAGEL4, BioCyc, KEEG, and InterSPPI) was used to predict the most important characteristics related to strains probiose. Comparative genomics analyses revealed that probiotic strains shared essential genes related to acid and bile stress response and antimicrobial activity. Other standard features shared by these strains are surface layer proteins and extracellular proteins-encoding genes, with high adhesion profiles that interacted with human proteins of the inflammatory signaling pathways (TLR2/4-MAPK, TLR2/4-NF-κB, and NOD-like receptors). Among these, the PrtB serine protease appears to be a strong candidate responsible for the anti-inflammatory properties reported for these strains. Furthermore, genes with high proteolytic and metabolic activity able to produce beneficial metabolites, such as acetate, bioactive peptides, and B-complex vitamins were also identified. These findings suggest that these proteins can be essential in biological mechanisms related to probiotics' beneficial effects of these strains in the host.
益生菌是对健康有益的微生物，主要具有免疫调节和抗炎特性。[具体菌种名称]是乳制品行业常用的一种细菌，其对宿主健康的益处已有报道。本研究分析了九株具有益生菌特性的[具体菌种名称]菌株的核心基因组，重点关注与其宿主健康益处相关的基因。为此，采用了包括多种软件和数据库（BPGA、SPAAN、BAGEL4、BioCyc、KEGG和InterSPPI）的组合方法来预测与[具体菌种名称]菌株益生菌特性相关的最重要特征。比较基因组学分析表明，[具体菌种名称]益生菌菌株共享与酸和胆汁应激反应以及抗菌活性相关的必需基因。这些菌株共有的其他标准特征是表层蛋白和细胞外蛋白编码基因，具有与炎症信号通路（TLR2/4-MAPK、TLR2/4-NF-κB和NOD样受体）的人类蛋白相互作用的高粘附特性。其中，PrtB丝氨酸蛋白酶似乎是这些菌株抗炎特性的有力候选因素。此外，还鉴定出具有高蛋白水解和代谢活性、能够产生有益代谢物（如乙酸盐、生物活性肽和B族复合维生素）的基因。这些发现表明，这些蛋白质在与这些菌株在宿主中益生菌有益作用相关的生物学机制中可能至关重要。
