van Kamp G J, van Bezu J S, Mulder C
Department of Clinical Chemistry, Free University Hospital, Amsterdam, The Netherlands.
Ann Clin Biochem. 1989 Sep;26 ( Pt 5):427-9. doi: 10.1177/000456328902600509.
We describe a fast, simple and sensitive microtiter scale competitive ELISA for the determination of urinary albumin. All reagents are commercially available. In the standard procedure, a minimum concentration of 0.8 mg albumin/L can be measured, although, applying another dilution, 0.04 mg/L can be detected. Within-batch coefficient of variation was 6.9% and 5.6% (at 1:20 and 1:50 dilution, respectively); between-batch variation was 8.6% and 5.6% respectively. The influence of urine pH and other urine components is minimised in the assay diluting with a casein-containing buffer.
我们描述了一种用于测定尿白蛋白的快速、简单且灵敏的微量滴定板竞争性酶联免疫吸附测定法。所有试剂均有市售。在标准程序中，最低可检测到的白蛋白浓度为0.8毫克/升，不过，采用另一种稀释方法时，可检测到0.04毫克/升。批内变异系数分别为6.9%和5.6%（分别在1:20和1:50稀释时）；批间变异分别为8.6%和5.6%。在用含酪蛋白的缓冲液进行测定稀释时，尿液pH值和其他尿液成分的影响被降至最低。
