Wu Zhong-Nan, Zhang Yu-Bo, Chen Neng-Hua, Li Mo-Jiao, Li Man-Mei, Tang Wei, Zhuang Ling, Li Yao-Lan, Wang Guo-Cai
Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China.
Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China.
Phytochemistry. 2017 May;137:81-86. doi: 10.1016/j.phytochem.2017.01.020. Epub 2017 Feb 5.
Seven sesquiterpene lactones, 8-O-methacryloylelephanpane, 2,4-bis-O-methyl-8-O-methacryloylelephanpane, 4-O-ethyl-8-O-methacryloylelephanpane, 8-O-methacryloylisoelephanpane, 2-O-demethyltomenphantopin C, molephantin A, molephantin B, along with ten known ones, were isolated from Elephantopus mollis (Asteraceae). Their structures were elucidated by extensive analysis of spectroscopic data (IR, UV, HRESIMS, 1D and 2D NMR). The isolates were evaluated for their anti-inflammatory activities on LPS-stimulated RAW 264.7 cells, and all tested compounds exhibited potent anti-inflammatory effects with IC values of 0.57 ± 0.17 to 14.34 ± 1.61 μM, except that compound tomenphantopin C exhibited moderate anti-inflammatory activity with IC values of 59.97 ± 1.53 μM.
从地胆草（菊科）中分离得到了7种倍半萜内酯，8 - O - 甲基丙烯酰基紫铆烷、2,4 - 二 - O - 甲基 - 8 - O - 甲基丙烯酰基紫铆烷、4 - O - 乙基 - 8 - O - 甲基丙烯酰基紫铆烷、8 - O - 甲基丙烯酰基异紫铆烷、2 - O - 去甲基托曼芬平C、莫莱芬平A、莫莱芬平B，以及10种已知化合物。通过对光谱数据（红外光谱、紫外光谱、高分辨电喷雾电离质谱、一维和二维核磁共振谱）的广泛分析确定了它们的结构。对这些分离物在脂多糖刺激的RAW 264.7细胞上的抗炎活性进行了评估，除托曼芬平C化合物表现出中等抗炎活性（IC值为59.97±1.53 μM）外，所有测试化合物均表现出强效抗炎作用，IC值为0.57±0.17至14.34±1.61 μM。
