Department of Chemical Engineering, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul, Republic of Korea.
Department of Chemical and Biomolecular Engineering, Yonsei University, 50, Yonsei-ro, Seodaemun-gu, Seoul, Republic of Korea.
N Biotechnol. 2017 Jul 25;37(Pt B):194-199. doi: 10.1016/j.nbt.2017.02.001. Epub 2017 Feb 5.
We exploited the emerging potential of gene therapy strategies to design a powerful therapeutic system that combines two key components-AAV vector and [6]-gingerol. In this study, we created an AAV2 construct expressing the proapoptotic protein BIM, which uses HSPG as its primary receptor, to target HSPG-overexpressing melanoma cells. This combination treatment showed promising results in vitro, inducing apoptosis in human melanoma cells. This new platform technology will make a significant contribution to numerous therapeutic applications, most notably for melanoma, including overcoming resistance to conventional anticancer therapies.
我们利用基因治疗策略的新兴潜力，设计了一种强大的治疗系统，该系统结合了两个关键组件——AAV 载体和[6]-姜酚。在这项研究中，我们构建了一个表达促凋亡蛋白 BIM 的 AAV2 载体，该蛋白主要利用 HSPG 作为其受体，以靶向 HSPG 过表达的黑色素瘤细胞。这种联合治疗在体外显示出了有前景的结果，能诱导人黑色素瘤细胞凋亡。这项新的平台技术将为众多治疗应用做出重要贡献，特别是对黑色素瘤，包括克服对传统抗癌疗法的耐药性。
