Gheri G, Gheri Bryk S
Arch Ital Anat Embriol. 1989 Jan-Mar;94(1):71-83.
The histochemical characteristics of colonic epithelial mucins were investigated in the chick embryo. At the 14th day of incubation it was possible to demonstrate the presence of glycogen. At the 15th day a few epithelial cells showed the presence of neutral and sialylated mucins. On the 16th day, also sulfated secretory material was detectable together with neutral and sialylated mucins in cells with the typical shape of goblet cells. From the 17th day to the 20th day of incubation the two types of acid mucins appeared in some cells to be placed in distinct zones of the supranuclear cytoplasm. At the 21st day, neutral, sialylated and sulfated mucins were all present in the majority of goblet cells, which were found mainly in the epithelium lining the crypts.
在鸡胚中研究了结肠上皮粘蛋白的组织化学特征。在孵化第14天时，可以证明糖原的存在。在第15天时，一些上皮细胞显示存在中性和唾液酸化粘蛋白。在第16天时，在具有典型杯状细胞形状的细胞中，除了中性和唾液酸化粘蛋白外，还可检测到硫酸化分泌物质。从孵化第17天到第20天，两种酸性粘蛋白出现在一些细胞中，位于核上细胞质的不同区域。在第21天时，中性、唾液酸化和硫酸化粘蛋白在大多数杯状细胞中均有存在，这些杯状细胞主要位于隐窝内衬的上皮中。
