Gheri G, Bryk S G, Orlandini G E
Department of Human Anatomy and Histology, University of Florence, Italy.
Histochemistry. 1988;88(3-6):519-24. doi: 10.1007/BF00570319.
Mucosubstances of epithelial cells of the chick embryo gallbladder were investigated by histochemical methods from days 12-21 of incubation (stages 38-46). On incubation day 14, only neutral mucins were detected; on day 15, neutral and sulfated mucosubstances were observed in the epithelial cells that invaginate the underlying mesenchyme. In the same sites, at day 16 of incubation, neutral, carboxylated and sulfated mucins were seen. From the 17th day of incubation until hatching, neutral, carboxylated and sulfated mucosubstances were present in the surface cells and in the cells lining the epithelial invaginations. During this period, the chemical characteristics of the secretory material are similar to those observed by Yamada and Hoshino (1972) in the fowl.
通过组织化学方法，对孵化第12至21天（第38至46阶段）鸡胚胆囊上皮细胞的黏液物质进行了研究。在孵化第14天，仅检测到中性黏蛋白；在第15天，在向下方间充质内陷的上皮细胞中观察到中性和硫酸化黏液物质。在孵化第16天，在相同部位可见中性、羧化和硫酸化黏蛋白。从孵化第17天直至孵化，表面细胞以及上皮内陷内衬细胞中均存在中性、羧化和硫酸化黏液物质。在此期间，分泌物质的化学特性与Yamada和Hoshino（1972年）在禽类中观察到的相似。
