鸡胚前胃上皮细胞黏蛋白的组织化学特征

Sgambati E, Bryk S G, Gheri G

Department of Human Anatomy and Histology, University of Florence Italy.

Ital J Anat Embryol. 1995 Apr-Jun;100(2):83-97.

The histochemical characteristics of the epithelial mucins, which are produced by the lining epithelium, the superficial glands and the compound glands of the proventriculus were investigated in the chick embryo from the 7th day of incubation to hatching. Results showed that the time of appearance of the carboxylated, sulphated and neutral mucosubstances differed at the three investigated sites. From the 13th to the 19th day of incubation, intense production of the various mucins was detected at the three sites. However, the modality of intracytoplasmatic storage and the production of mucins in both the cells of the lining epithelium and the superficial glands appeared to differ from those observed in the cells of the compound glands. At the 20th and 21st day, the mucins were primarily produced by the lining epithelium and the superficial glands, whereas production diminished in the compound glands. Furthermore, from the 7th to the 17th day of incubation, the cells of the principal ducts of the compound glands showed the same histochemical characteristics and modality of production as those of the glandular cells of the compound glands. On the other hand, from the 18th day to hatching, these cells assumed the same characteristics as those of the lining epithelium and of the superficial glands.

对孵化第7天至孵化出壳的鸡胚前胃内衬上皮、浅表腺体和复合腺产生的上皮黏蛋白的组织化学特征进行了研究。结果表明，在所研究的三个部位，羧化、硫酸化和中性黏液物质的出现时间有所不同。在孵化的第13天至第19天，在这三个部位均检测到各种黏蛋白的大量产生。然而，内衬上皮细胞和浅表腺细胞中黏蛋白的胞质内储存方式和产生情况似乎与复合腺细胞中观察到的不同。在第20天和第21天，黏蛋白主要由内衬上皮和浅表腺体产生，而复合腺中的产生量减少。此外，在孵化的第7天至第17天，复合腺主导管的细胞显示出与复合腺腺细胞相同的组织化学特征和产生方式。另一方面，从第18天到孵化出壳，这些细胞呈现出与内衬上皮和浅表腺体相同的特征。