Unité de Physiopathologie Clinique Centre Hospitalier Universitaire Vaudois and University of Lausanne, Bugnon 7a, 1005 Lausanne, Switzerland.
Service de Médecine Intensive Adulte, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Switzerland.
Sci Rep. 2017 Feb 9;7:41936. doi: 10.1038/srep41936.
Brain Natriuretic Peptide (BNP) injections in adult "healthy" or infarcted mice led to increased number of non-myocyte cells (NMCs) expressing the nuclear transcription factor Nkx2.5. The aim of this study was to identify the nature of the cells able to respond to BNP as well as the signaling pathway involved. BNP treatment of neonatal mouse NMCs stimulated Sca-1 cell proliferation. The Sca-1 cells were characterized as being a mixed cell population involving fibroblasts and multipotent precursor cells. Thus, BNP treatment led also to increased number of Sca-1 cells expressing Nkx2.5, in Sca-1 cell cultures in vitro and in vivo, in the hearts of neonatal and adult infarcted mice. Whereas BNP induced Sca-1 cell proliferation via NPR-B receptor and protein kinase G activation, CNP stimulated Sca-1 cell proliferation via NPR-B and a PKG-independent mechanism. We highlighted here a new role for the natriuretic peptide receptor B which was identified as a target able to modulate the proliferation of the Sca-1 cells. The involvement of NPR-B signaling in heart regeneration has, however, to be further investigated.
脑利钠肽（BNP）在成年“健康”或梗死小鼠中的注射导致表达核转录因子 Nkx2.5 的非心肌细胞（NMC）数量增加。本研究的目的是鉴定能够对 BNP 作出反应的细胞的性质以及涉及的信号通路。BNP 处理新生小鼠 NMC 可刺激 Sca-1 细胞增殖。Sca-1 细胞的特征是一种混合细胞群，涉及成纤维细胞和多能前体细胞。因此，BNP 处理还导致 Sca-1 细胞中表达 Nkx2.5 的细胞数量增加，无论是在体外和体内的 Sca-1 细胞培养物中，还是在新生和成年梗死小鼠的心脏中。虽然 BNP 通过 NPR-B 受体和蛋白激酶 G 激活诱导 Sca-1 细胞增殖，但 CNP 通过 NPR-B 和一种不依赖于 PKG 的机制刺激 Sca-1 细胞增殖。我们在这里强调了利钠肽受体 B 的一个新作用，该受体被鉴定为能够调节 Sca-1 细胞增殖的靶标。然而，需要进一步研究 NPR-B 信号在心脏再生中的作用。
