Yan Lulu, Su Jiaqi, Wang Zhaoping, Yan Xiwu, Yu Ruihai, Ma Peizhen, Li Yangchun, Du Junpeng
Fisheries College, Ocean University of China, Qingdao, Shandong, China.
The Key Lab of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, China.
PLoS One. 2017 Feb 9;12(2):e0171483. doi: 10.1371/journal.pone.0171483. eCollection 2017.
Hybrid oysters often show heterosis in growth rate, weight, survival and adaptability to extremes of salinity. Oysters have also been used as model organisms to study the evolution of host-defense system. To gain comprehensive knowledge about various physiological processes in hybrid oysters under low salinity stress, we performed transcriptomic analysis of gill tissue of Crassostrea sikamea ♀ × Crassostrea angulata♂ hybrid using the deep-sequencing platform Illumina HiSeq. We exploited the high-throughput technique to delineate differentially expressed genes (DEGs) in oysters maintained in hypotonic conditions. A total of 199,391 high quality unigenes, with average length of 644 bp, were generated. Of these 35 and 31 genes showed up- and down-regulation, respectively. Functional categorization and pathway analysis of these DEGs revealed enrichment for immune mechanism, apoptosis, energy metabolism and osmoregulation under low salinity stress. The expression patterns of 41 DEGs in hybrids and their parental species were further analyzed by quantitative real-time PCR (qRT-PCR). This study will serve as a platform for subsequent gene expression analysis regarding environmental stress. Our findings will also provide valuable information about gene expression to better understand the immune mechanism, apoptosis, energy metabolism and osmoregulation in hybrid oysters under low salinity stress.
杂交牡蛎在生长速度、体重、存活率以及对极端盐度的适应性方面常常表现出杂种优势。牡蛎也被用作研究宿主防御系统进化的模式生物。为了全面了解低盐度胁迫下杂交牡蛎的各种生理过程，我们使用Illumina HiSeq深度测序平台对近江牡蛎♀×香港巨牡蛎♂杂交种的鳃组织进行了转录组分析。我们利用高通量技术来描绘在低渗条件下饲养的牡蛎中差异表达基因（DEG）。共产生了199,391个高质量单基因，平均长度为644 bp。其中，分别有35个和31个基因上调和下调。对这些DEG进行功能分类和通路分析，发现在低盐度胁迫下免疫机制、细胞凋亡（凋亡）、能量代谢和渗透调节方面存在富集。通过定量实时PCR（qRT-PCR）进一步分析了41个DEG在杂种及其亲本物种中的表达模式。本研究将作为后续关于环境胁迫的基因表达分析的平台。我们的研究结果还将提供有关基因表达的有价值信息，以便更好地了解低盐度胁迫下杂交牡蛎的免疫机制、细胞凋亡、能量代谢和渗透调节。
