Department of Oncology, The Affiliated Hospital of Southwest Medical University, Southwest Medical University, Luzhou 646000, PR China.
Department of Science and Technology, Southwest Medical University, Luzhou 646000, PR China.
Mater Sci Eng C Mater Biol Appl. 2017 Apr 1;73:537-543. doi: 10.1016/j.msec.2016.12.116. Epub 2016 Dec 24.
Magnetic nanoparticles have been one of the most attractive nanomaterials for various biomedical applications including magnetic resonance imaging (MRI), diagnostic contrast enhancement, magnetic cell separation, and targeted drug delivery. Three-dimensional (3-D) fibrous scaffolds have broad application prospects in the biomedical field, such as drug delivery and tissue engineering. In this work, a novel three-dimensional composite membrane composed of the tri-block copolymer poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, PCEC) and magnetic iron oxide nanoparticles (FeO NPs) were fabricated using electrospinning technology. The physico-chemical properties of the PCEC/FeO membranes were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and differential scanning calorimetry (DSC). Morphological observation using scanning electron microscopy (SEM) showed that the composite fibers containing 5% FeO nanoparticles had a diameter of 250nm. In vitro cell culture of NIH 3T3 cells on the PCEC/FeO membranes showed that the PCEC/FeO fibers might be a suitable scaffold for cell adhesion. Moreover, MTT analysis also demonstrated that the membranes possessed lower cytotoxicity. Therefore, this study revealed that the magnetic PCEC/FeO fibers might have great potential for using in skin tissue engineering.
磁性纳米粒子是最具吸引力的纳米材料之一，可应用于各种生物医学领域，包括磁共振成像（MRI）、诊断对比增强、磁性细胞分离和靶向药物输送。三维（3-D）纤维支架在生物医学领域具有广泛的应用前景，如药物输送和组织工程。在这项工作中，使用静电纺丝技术制备了一种由三嵌段共聚物聚（ε-己内酯）-聚乙二醇-聚（ε-己内酯）（PCL-PEG-PCL，PCEC）和磁性氧化铁纳米粒子（FeO NPs）组成的新型三维复合膜。通过傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）和差示扫描量热法（DSC）研究了 PCEC/FeO 膜的物理化学性质。扫描电子显微镜（SEM）的形貌观察表明，含有 5%FeO 纳米粒子的复合纤维直径为 250nm。NIH 3T3 细胞在 PCEC/FeO 膜上的体外细胞培养表明，PCEC/FeO 纤维可能是一种适合细胞黏附的支架。此外，MTT 分析也表明该膜具有较低的细胞毒性。因此，本研究表明，磁性 PCEC/FeO 纤维可能在皮肤组织工程中有很大的应用潜力。
