Veres Tamás, Voniatis Constantinos, Molnár Kristóf, Nesztor Dániel, Fehér Daniella, Ferencz Andrea, Gresits Iván, Thuróczy György, Márkus Bence Gábor, Simon Ferenc, Nemes Norbert Marcell, García-Hernández Mar, Reiniger Lilla, Horváth Ildikó, Máthé Domokos, Szigeti Krisztián, Tombácz Etelka, Jedlovszky-Hajdu Angela
Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University, 1089 Budapest, Hungary.
Department of Surgery, Transplantation and Gastroenterology, Semmelweis University, 1082 Budapest, Hungary.
Nanomaterials (Basel). 2022 Apr 26;12(9):1476. doi: 10.3390/nano12091476.
When exposed to an alternating magnetic field, superparamagnetic nanoparticles can elicit the required hyperthermic effect while also being excellent magnetic resonance imaging (MRI) contrast agents. Their main drawback is that they diffuse out of the area of interest in one or two days, thus preventing a continuous application during the typical several-cycle multi-week treatment. To solve this issue, our aim was to synthesise an implantable, biodegradable membrane infused with magnetite that enabled long-term treatment while having adequate MRI contrast and hyperthermic capabilities. To immobilise the nanoparticles inside the scaffold, they were synthesised inside hydrogel fibres. First, polysuccinimide (PSI) fibres were produced by electrospinning and crosslinked, and then, magnetitc iron oxide nanoparticles (MIONs) were synthesised inside and in-between the fibres of the hydrogel membranes with the well-known co-precipitation method. The attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR) investigation proved the success of the chemical synthesis and the presence of iron oxide, and the superconducting quantum interference device (SQUID) study revealed their superparamagnetic property. The magnetic hyperthermia efficiency of the samples was significant. The given alternating current (AC) magnetic field could induce a temperature rise of 5 °C (from 37 °C to 42 °C) in less than 2 min even for five quick heat-cool cycles or for five consecutive days without considerable heat generation loss in the samples. Short-term (1 day and 7 day) biocompatibility, biodegradability and MRI contrast capability were investigated in vivo on Wistar rats. The results showed excellent MRI contrast and minimal acute inflammation.
当暴露于交变磁场时，超顺磁性纳米颗粒可以引发所需的热疗效果，同时也是出色的磁共振成像（MRI）造影剂。它们的主要缺点是在一到两天内就会扩散出感兴趣的区域，从而无法在典型的数周期数周治疗过程中持续应用。为了解决这个问题，我们的目标是合成一种注入磁铁矿的可植入、可生物降解的膜，使其能够进行长期治疗，同时具备足够的MRI造影和热疗能力。为了将纳米颗粒固定在支架内，我们在水凝胶纤维内部合成了它们。首先，通过静电纺丝制备聚琥珀酰亚胺（PSI）纤维并进行交联，然后，采用著名的共沉淀法在水凝胶膜的纤维内部和纤维之间合成磁性氧化铁纳米颗粒（MIONs）。衰减全反射傅里叶变换红外光谱（ATR-FTIR）研究证明了化学合成的成功以及氧化铁的存在，超导量子干涉装置（SQUID）研究揭示了它们的超顺磁性。样品的磁热疗效率显著。即使进行五个快速加热-冷却循环或连续五天，给定的交变电流（AC）磁场也能在不到2分钟的时间内使温度从37°C升高到42°C，且样品中没有明显的热生成损失。在Wistar大鼠体内研究了短期（1天和7天）的生物相容性、生物降解性和MRI造影能力。结果显示出出色的MRI造影效果和最小的急性炎症反应。
