Marmara University, Department of Chemistry, 34722, Goztepe, Istanbul, Turkey.
Marmara University, Department of Chemistry, 34722, Goztepe, Istanbul, Turkey.
Mater Sci Eng C Mater Biol Appl. 2017 Apr 1;73:569-578. doi: 10.1016/j.msec.2016.12.082. Epub 2016 Dec 23.
In this study, zwitterionic phosphorylcholine grafted electrospun chitosan fiber was accomplished in three steps: (1) Azide groups on the chitosan were regioselectively replaced with hydroxyl side group and then the product was electrospun. (2) Chitosan based macroinitiator was prepared using an azide-alkyne click reaction from azide-functionalized electrospun chitosan fiber. (3) Poly(2-methacryloyloxyethyl phosphorylcholine) (MPC) was grafted onto the electrospun chitosan fiber by atom transfer radical polymerization (ATRP) in order to enhance cellular viability and proliferation of 3T3, ECV and Saos. The structure of surface modified chitosan was characterized by Fourier transform infrared spectrometer (FT-IR) and H nuclear magnetic resonance (H NMR). The surface morphology of the nanofibers was investigated by scanning electron microscope (SEM). In-vitro cellular attachment and spreading experiments of 3T3, ECV304 and Saos were performed on electrospun chitosan fibers in the presence and the absence of MPC grafting. Poly(MPC) grafted electrospun fiber showed an excellent performance due to phosphorylcholine groups mimicking the natural phospholipid.
在这项研究中，通过三个步骤完成了两性离子磷酸胆碱接枝静电纺丝壳聚糖纤维：（1）壳聚糖上的叠氮基团被区域选择性地取代为羟基侧基，然后将产物进行静电纺丝。（2）通过叠氮 - 炔点击反应，从叠氮功能化的静电纺丝壳聚糖纤维制备壳聚糖基大分子引发剂。（3）通过原子转移自由基聚合（ATRP）将聚（2-甲基丙烯酰氧乙基磷酸胆碱）（MPC）接枝到静电纺丝壳聚糖纤维上，以提高 3T3、ECV 和 Saos 的细胞活力和增殖。表面改性壳聚糖的结构通过傅里叶变换红外光谱（FT-IR）和 H 核磁共振（H NMR）进行了表征。通过扫描电子显微镜（SEM）研究了纳米纤维的表面形态。在存在和不存在 MPC 接枝的情况下，在静电纺丝壳聚糖纤维上进行了 3T3、ECV304 和 Saos 的体外细胞附着和扩散实验。由于磷酸胆碱基团模拟天然磷脂，因此接枝了聚（MPC）的静电纺丝纤维表现出优异的性能。
