通过原子转移自由基聚合法将漆酶固定在改性聚氨酯/还原氧化石墨烯纳米纤维上及其在去除双酚A中的应用。
Immobilization of laccase onto modified PU/RC nanofiber via atom transfer radical polymerization method and application in removal of bisphenol A.
作者信息
Li Xin, Li Dawei, Lv Pengfei, Hu Jinyan, Feng Quan, Wei Qufu
机构信息
Key Laboratory of Eco-Textiles, Ministry of Education Jiangnan University Jiangsu Province Wuxi P. R. China.
Key Laboratory of Textile Fabric Anhui Polytechnic University Wuhu Anhui P. R. China.
出版信息
Eng Life Sci. 2019 Nov 4;19(11):815-824. doi: 10.1002/elsc.201900075. eCollection 2019 Nov.
In this study, 2-hydroxyethyl methacrylate (HEMA) was used as the monomers for surface grafting on electrospun PU/RC nanofiber membrane via atom transfer radical polymerization (ATRP) method, and the PU/RC-poly(HEMA) nanofiber membrane was investigated as a carrier for LAC. Free and immobilized LAC was characterized, and efficiency of bisphenol A (BPA) removal was determined. The results indicated that the PU/RC-poly(HEMA)-LAC showed relatively higher pH stability, temperature stability, and storage stability than free and PU/RC-LAC; moreover, more than 60% of the PU/RC-poly(HEMA)-LAC activity was retained after 10 cycles of ABTS treatment. Notably, the BPA removal efficiency of PU/RC-poly(HEMA)-LAC membrane generally ranged from 87.3 to 75.4% for the five cycles. Therefore, the PU/RC-poly(HEMA) nanofiber membrane has great potential as a carrier for the LAC immobilization for various industrial applications and bioremediation.
在本研究中，甲基丙烯酸2-羟乙酯（HEMA）被用作单体，通过原子转移自由基聚合（ATRP）法在静电纺丝的PU/RC纳米纤维膜上进行表面接枝，并且对PU/RC-聚（HEMA）纳米纤维膜作为漆酶（LAC）的载体进行了研究。对游离态和固定化的漆酶进行了表征，并测定了双酚A（BPA）的去除效率。结果表明，与游离态和PU/RC-漆酶相比，PU/RC-聚（HEMA）-漆酶表现出相对更高的pH稳定性、温度稳定性和储存稳定性；此外，经过10次ABTS处理循环后，PU/RC-聚（HEMA）-漆酶仍保留了超过60%的活性。值得注意的是，PU/RC-聚（HEMA）-漆酶膜在五个循环中对BPA的去除效率一般在87.3%至75.4%之间。因此，PU/RC-聚（HEMA）纳米纤维膜作为漆酶固定化载体在各种工业应用和生物修复方面具有巨大潜力。
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