Applied Science and Technology Department, Institute of Materials Physics and Engineering, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy.
Department of Chemistry, University of Milan, Via Golgi 19, 20133 Milan, Italy.
Mater Sci Eng C Mater Biol Appl. 2017 Apr 1;73:778-787. doi: 10.1016/j.msec.2016.12.105. Epub 2016 Dec 23.
Ferrimagnetic glass-ceramics containing magnetite crystals were developed for hyperthermia applications of solid neoplastic tissue. The present work is focused on in vitro evaluation of the biocompatibility of these materials, before and after soaking in a simulated body fluid (SBF). X-ray diffraction, scanning electron microscopy, atomic absorption spectrophotometry, X-ray photoelectron spectrometry and pH measurements were employed in glass-ceramic characterisation. The free-radical mediated reactivity of the glass-ceramic was evaluated by Electron Paramagnetic Resonance (EPR) spin trapping. Cell adhesion and proliferation tests were carried out by using 3T3 murine fibroblasts. Cytotoxicity was performed by qualitative evaluation of human bone osteosarcoma cells U2OS cell line. The results show that almost two times more 3T3 cells proliferated on the samples pre-treated in SBF, compared with the untreated specimens. Moreover a decrease of confluence was observed at 48 and 72h for U2OS cells exposed to the untreated glass-ceramic, while the powder suspensions of glass-ceramic pre-treated in SBF did not influence the cell morphology up to 72h of exposition. The untreated glass-ceramic exhibited Fenton-like reactivity, as well as reactivity towards formate molecule. After pre-treatment with SBF the reactivity towards formate was completely suppressed. The concentration of iron released into the SBF solution was below 0.1ppm at 37°C, during one month of soaking. The different in vitro behaviour of the samples before and after SBF treatment has been correlated to the bioactive glass-ceramic surface modifications as detected by morphological, structural and compositional analyses.
研制了含有磁铁矿晶体的铁磁性微晶玻璃，用于固态肿瘤组织的热疗应用。本工作主要关注这些材料在浸泡于模拟体液（SBF）前后的体外生物相容性评估。采用 X 射线衍射、扫描电子显微镜、原子吸收分光光度法、X 射线光电子能谱和 pH 测量对微晶玻璃进行了表征。通过电子顺磁共振（EPR）自旋捕获评估了微晶玻璃的自由基介导反应性。通过使用 3T3 鼠成纤维细胞进行细胞黏附和增殖试验。通过定性评估人骨肉瘤细胞 U2OS 细胞系进行细胞毒性试验。结果表明，与未经处理的样品相比，在 SBF 预处理后的样品上，3T3 细胞的增殖速度快了近两倍。此外，暴露于未经处理的微晶玻璃的 U2OS 细胞在 48 和 72 小时时汇合度降低，而经 SBF 预处理的微晶玻璃粉末悬浮液在 72 小时的暴露时间内对细胞形态没有影响。未经处理的微晶玻璃表现出类 Fenton 反应性，以及对甲酸盐分子的反应性。经过 SBF 预处理后，对甲酸盐的反应性完全被抑制。在 37°C 下浸泡一个月，释放到 SBF 溶液中的铁浓度低于 0.1ppm。在 SBF 处理前后，样品的体外行为不同，这与通过形态、结构和成分分析检测到的生物活性微晶玻璃表面改性有关。
