Mondal Sudip, Manivasagan Panchanathan, Bharathiraja Subramaniyan, Santha Moorthy Madhappan, Nguyen Van Tu, Kim Hye Hyun, Nam Seung Yun, Lee Kang Dae, Oh Junghwan
Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Korea.
Interdisciplinary Program of Biomedical Mechanical and Electrical Engineering, Pukyong National University, Busan 48513, Korea.
Nanomaterials (Basel). 2017 Dec 4;7(12):426. doi: 10.3390/nano7120426.
Targeting cancer cells without injuring normal cells is the prime objective in treatment of cancer. In this present study, solvothermal and wet chemical precipitation techniques were employed to synthesize iron oxide (IO), hydroxyapatite (HAp), and hydroxyapatite coated iron oxide (IO-HAp) nanoparticles for magnetic hyperthermia mediated cancer therapy. The synthesized well dispersed spherical IO-HAp nanoparticles, magnetite, and apatite phases were confirmed by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Field emission transmission electron microscopy (FETEM) with Energy Dispersive X-ray spectroscopy (EDS). The non-toxic behavior of synthesized IO-HAp nanoparticles was confirmed by cytotoxicity assay (Trypan blue and MTT assay). The synthesized nanoparticles revealed a remarkable magnetic saturation of 83.2 emu/g for IO and 40.6 emu/g for IO-HAp nanoparticles in presence of 15,000 Oe (1.5 T) magnetic field at room temperature (300 K). The magnetic hyperthermia study that was performed with IO-HAp nanoparticles showed an excellent hyperthermia effect (SAR value 85 W/g) over MG-63 osteosarcoma cells. The in vitro hyperthermia temperature (~45 °C) was reached within 3 min, which shows a very high efficiency and kills nearly all of the experimental MG-63 osteosarcoma cells within 30 min exposure. These results could potentially open new perceptions for biomaterials that are aimed for anti-cancer therapies based on magnetic hyperthermia.
在癌症治疗中，靶向癌细胞而不损伤正常细胞是首要目标。在本研究中，采用溶剂热法和湿化学沉淀技术合成了用于磁热疗介导癌症治疗的氧化铁（IO）、羟基磷灰石（HAp）和羟基磷灰石包覆氧化铁（IO-HAp）纳米颗粒。通过X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）以及配备能量色散X射线光谱（EDS）的场发射透射电子显微镜（FETEM），证实了合成的分散良好的球形IO-HAp纳米颗粒、磁铁矿相和磷灰石相。通过细胞毒性试验（台盼蓝和MTT试验）证实了合成的IO-HAp纳米颗粒的无毒行为。在室温（300 K）下，在15,000 Oe（1.5 T）磁场存在的情况下，合成的纳米颗粒显示出IO纳米颗粒的磁饱和度为83.2 emu/g，IO-HAp纳米颗粒的磁饱和度为40.6 emu/g。用IO-HAp纳米颗粒进行的磁热疗研究显示，对MG-63骨肉瘤细胞具有优异的热疗效果（SAR值85 W/g）。在3分钟内达到体外热疗温度（约45°C），这显示出非常高的效率，并且在30分钟的暴露时间内几乎杀死了所有实验性MG-63骨肉瘤细胞。这些结果可能为基于磁热疗的抗癌治疗生物材料开辟新的认识。
