补充顺式棕榈油酸对溃疡性结肠炎患者炎症及肝细胞核因子4γ、肝细胞核因子4α和白细胞介素6表达的影响。
Effect of Cis-palmitoleic acid supplementation on inflammation and expression of HNF4γ, HNF4α and IL6 in patients with ulcerative colitis.
作者信息
Bueno-Hernández Nallely, Sixtos-Alonso María S, Milke García María Del Pilar, Yamamoto-Furusho Jesús K
机构信息
Department of Gastroenterology, Inflammatory Bowel Disease Clinic, National Institute of Medical Sciences and Nutrition "Salvador Zubirán" (INCMNSZ), Mexico City, Mexico.
Gastroenterology Laboratory, National Institute of Medical Sciences and Nutrition "Salvador Zubirán" (INCMNSZ), Mexico City, Mexico.
出版信息
Minerva Gastroenterol Dietol. 2017 Sep;63(3):257-263. doi: 10.23736/S1121-421X.17.02367-4. Epub 2017 Feb 9.
BACKGROUND
Cis-palmitoleic acid (Omega-7 fatty acid) is a monounsaturated fatty acid (MUFA) associated with anti-inflammatory process through specific protein interactions such as HNF4γ and HNF4α, these two genes are related to the immune response in ulcerative colitis (UC) they may act as a mediator of anti-inflammatory action. The aim of this study was to evaluate the effect of Cis-palmitoleic acid supplementation on inflammatory activity and the expression of genes HNF4γ, HNF4α and IL6 in the colonic mucosa of patients with active UC.
METHODS
A double-blind, randomized, placebo-controlled pilot study was conducted in 20 patients with UC. A dose of 720 mg/day of Cis-palmitoleic acid was orally administered during 8 weeks and Mayo Clinic score was used for the assessment of clinical activity in UC before and after treatment with Cis-palmitoleic acid and placebo.
RESULTS
A total of 20 patients with UC were randomized to receive Cis-palmitoleic acid or placebo. Significant changes in the biochemical markers of inflammation were found in UC patients before and after treatment with Cis-palmitoleic acid vs. placebo such as total protein (P=0.02), hs-CRP (P=0.04) and ESR (P<0.05). The gene expression of HNF4γ and HNF4α were found to be increased in the Cis-palmitoleic acid group compared to placebo group (P=0.05 and P=0.07 respectively) as well as significant reduction upon IL6 expression in the Cis-palmitoleic acid group (P=0.005).
CONCLUSIONS
Cis-palmitoleic acid as co-adjuvant therapy for 8 weeks seems to decrease the inflammatory activity through the increased expression of HNF4α and HNF4γ in patients with UC.
背景
顺式棕榈油酸（欧米伽-7脂肪酸）是一种单不饱和脂肪酸（MUFA），通过与特定蛋白质相互作用（如HNF4γ和HNF4α）参与抗炎过程，这两个基因与溃疡性结肠炎（UC）的免疫反应相关，它们可能作为抗炎作用的介质。本研究旨在评估补充顺式棕榈油酸对活动性UC患者结肠黏膜炎症活性以及HNF4γ、HNF4α和IL6基因表达的影响。
方法
对20例UC患者进行了一项双盲、随机、安慰剂对照的试点研究。在8周内口服720毫克/天的顺式棕榈油酸，并使用梅奥诊所评分评估顺式棕榈油酸和安慰剂治疗前后UC的临床活性。
结果
共有20例UC患者被随机分为接受顺式棕榈油酸或安慰剂组。与安慰剂相比，顺式棕榈油酸治疗前后UC患者炎症生化标志物有显著变化，如总蛋白（P=0.02）、超敏C反应蛋白（P=0.04）和血沉（P<0.05）。与安慰剂组相比，顺式棕榈油酸组中HNF4γ和HNF4α的基因表达增加（分别为P=0.05和P=0.07），顺式棕榈油酸组中IL6表达也显著降低（P=0.005）。
结论
顺式棕榈油酸作为辅助治疗8周似乎通过增加UC患者HNF4α和HNF4γ的表达来降低炎症活性。
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