1,25-二羟维生素D3向钙三醇酸的靶细胞代谢。肾脏和骨骼中涉及24-氧化途径的证据。
Target cell metabolism of 1,25-dihydroxyvitamin D3 to calcitroic acid. Evidence for a pathway in kidney and bone involving 24-oxidation.
作者信息
Makin G, Lohnes D, Byford V, Ray R, Jones G
机构信息
Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.
出版信息
Biochem J. 1989 Aug 15;262(1):173-80. doi: 10.1042/bj2620173.
Abstract
1,25-dihydroxyvitamin D3 is converted to calcitroic acid before being excreted in the bile. Biosynthesis of calcitroic acid has been demonstrated in two target cells of vitamin D, in the kidney and the osteoblastic cell line UMR-106. Calcitroic acid was identified by combinations of h.p.l.c., u.v. spectroscopy and mass spectrometry. Evidence is presented that calcitroate is derived from the 24-oxidation pathway, possibly through the intermediate 24,25,26,27-tetranor-1,23-dihydroxyvitamin D3. The 24-oxidation pathway to calcitroic acid in bone cells is stimulated by 1,25-dihydroxyvitamin D3. The pathway in both bone cells and perfused kidney operates at physiological concentrations of substrate and appears to be capable of rapid clearance of the hormone.
摘要
1,25-二羟基维生素D3在经胆汁排泄之前会转化为钙三醇酸。在维生素D的两个靶细胞（肾脏和成骨细胞系UMR-106）中已证实了钙三醇酸的生物合成。通过高效液相色谱、紫外光谱和质谱联用鉴定出了钙三醇酸。有证据表明，钙三醇酸盐源自24-氧化途径，可能是通过中间产物24,25,26,27-四降-1,23-二羟基维生素D3。骨细胞中通向钙三醇酸的24-氧化途径受1,25-二羟基维生素D3刺激。骨细胞和灌注肾脏中的该途径在底物的生理浓度下起作用，并且似乎能够快速清除该激素。
相似文献
1
Target cell metabolism of 1,25-dihydroxyvitamin D3 to calcitroic acid. Evidence for a pathway in kidney and bone involving 24-oxidation.1,25-二羟维生素D3向钙三醇酸的靶细胞代谢。肾脏和骨骼中涉及24-氧化途径的证据。
Biochem J. 1989 Aug 15;262(1):173-80. doi: 10.1042/bj2620173.
2
Calcitroic acid, end product of renal metabolism of 1,25-dihydroxyvitamin D3 through C-24 oxidation pathway.骨化三醇酸，1,25-二羟基维生素D3经C-24氧化途径在肾脏代谢的终产物。
Biochemistry. 1989 Feb 21;28(4):1763-9. doi: 10.1021/bi00430a051.
3
Calcitroic acid is a major catabolic metabolite in the metabolism of 1 alpha-dihydroxyvitamin D(2).骨化酸是1α-二羟基维生素D（2）代谢中的主要分解代谢产物。
Arch Biochem Biophys. 2001 Aug 1;392(1):14-22. doi: 10.1006/abbi.2001.2419.
4
Isolation and identification of 1,23-dihydroxy-24,25,26,27-tetranorvitamin D3, a new metabolite of 1,25-dihydroxyvitamin D3 produced in rat kidney.1,23 - 二羟基 - 24,25,26,27 - 四降维生素D3的分离与鉴定，1,25 - 二羟基维生素D3在大鼠肾脏中产生的一种新代谢产物。
Biochemistry. 1987 Jan 13;26(1):324-31. doi: 10.1021/bi00375a045.
5
1alpha,25-dihydroxy-16-ene-23-yne-vitamin D3 and 1alpha,25-dihydroxy-16-ene-23-yne-20-epi-vitamin D3: analogs of 1alpha,25-dihydroxyvitamin D3 that resist metabolism through the C-24 oxidation pathway are metabolized through the C-3 epimerization pathway.1α,25 - 二羟基 - 16 - 烯 - 23 - 炔 - 维生素D3和1α,25 - 二羟基 - 16 - 烯 - 23 - 炔 - 20 - 表 - 维生素D3：1α,25 - 二羟基维生素D3的类似物，它们通过C - 24氧化途径抵抗代谢，而是通过C - 3差向异构化途径代谢。
Arch Biochem Biophys. 2000 Nov 15;383(2):197-205. doi: 10.1006/abbi.2000.2074.
6
Rat cytochrome P450C24 (CYP24) does not metabolize 1,25-dihydroxyvitamin D2 to calcitroic acid.大鼠细胞色素P450C24（CYP24）不会将1,25 - 二羟基维生素D2代谢为钙三醇酸。
J Cell Biochem. 2003 Feb 1;88(2):282-5. doi: 10.1002/jcb.10359.
7
1,25-Dihydroxyvitamin D3 metabolism in a human osteosarcoma cell line and human bone cells.
J Bone Miner Res. 1990 Jun;5(6):597-608. doi: 10.1002/jbmr.5650050609.
8
Characterization of the metabolic pathway of 1,25-dihydroxy-16-ene vitamin D3 in rat kidney by on-line high performance liquid chromatography-electrospray tandem mass spectrometry.利用在线高效液相色谱-电喷雾串联质谱法对大鼠肾脏中1,25-二羟基-16-烯维生素D3代谢途径的表征
Biochem Pharmacol. 1995 Apr 18;49(8):1099-710. doi: 10.1016/0006-2952(95)98507-6.
9
(23S)-1,23,25-Trihydroxyvitamin D3: its biologic activity and role in 1 alpha,25-dihydroxyvitamin D3 26,23-lactone biosynthesis.（23S）-1,23,25-三羟基维生素D3：其生物学活性及在1α,25-二羟基维生素D3 26,23-内酯生物合成中的作用。
Biochemistry. 1984 Aug 14;23(17):3973-9. doi: 10.1021/bi00312a026.
10
23-carboxy-24,25,26,27-tetranorvitamin D3 (calcioic acid) and 24-carboxy-25,26,27-trinorvitamin D3 (cholacalcioic acid): end products of 25-hydroxyvitamin D3 metabolism in rat kidney through C-24 oxidation pathway.23-羧基-24,25,26,27-四去甲维生素D3（钙酸）和24-羧基-25,26,27-三去甲维生素D3（胆钙酸）：大鼠肾脏中25-羟维生素D3经C-24氧化途径代谢的终产物。
Arch Biochem Biophys. 2006 Nov 1;455(1):18-30. doi: 10.1016/j.abb.2006.08.021. Epub 2006 Sep 15.
引用本文的文献
1
Anticancer Activity of Vitamin D, Lumisterol and Selected Derivatives against Human Malignant Melanoma Cell Lines.维生素 D、路美替醇及其选定衍生物对人恶性黑色素瘤细胞系的抗癌活性。
Int J Mol Sci. 2024 Oct 10;25(20):10914. doi: 10.3390/ijms252010914.
2
Physiology of Vitamin D-Focusing on Disease Prevention.维生素 D 的生理学——关注疾病预防。
Nutrients. 2024 May 29;16(11):1666. doi: 10.3390/nu16111666.
3
Phenotype of Idiopathic Infantile Hypercalcemia Associated with the Heterozygous Pathogenic Variant of and .与 和 的杂合致病变异相关的特发性婴儿高钙血症的表型
Children (Basel). 2023 Oct 17;10(10):1701. doi: 10.3390/children10101701.
4
Involucrin Modulates Vitamin D Receptor Activity in the Epidermis.桥粒芯糖蛋白调节表皮中维生素 D 受体的活性。
J Invest Dermatol. 2023 Jun;143(6):1052-1061.e3. doi: 10.1016/j.jid.2022.12.009. Epub 2023 Jan 13.
5
Diagnostic Aspects of Vitamin D: Clinical Utility of Vitamin D Metabolite Profiling.维生素D的诊断要点：维生素D代谢产物分析的临床应用
JBMR Plus. 2021 Dec 3;5(12):e10581. doi: 10.1002/jbm4.10581. eCollection 2021 Dec.
6
Overlapping Phenotypes Associated With , , and Mutations: A Cohort Study of Patients With Hypersensitivity to Vitamin D.与 、 、 突变相关的重叠表型：维生素 D 过敏患者的队列研究。
Front Endocrinol (Lausanne). 2021 Oct 13;12:736240. doi: 10.3389/fendo.2021.736240. eCollection 2021.
7
Biological evaluation and synthesis of calcitroic acid.生物评价与降钙素酸的合成。
Bioorg Chem. 2021 Nov;116:105310. doi: 10.1016/j.bioorg.2021.105310. Epub 2021 Aug 28.
8
24-Hydroxylase Deficiency Due to Sequence Variants: Comparison With Other Vitamin D-mediated Hypercalcemia Disorders.序列变异导致的24-羟化酶缺乏：与其他维生素D介导的高钙血症疾病的比较。
J Endocr Soc. 2021 Jul 2;5(9):bvab119. doi: 10.1210/jendso/bvab119. eCollection 2021 Sep 1.
9
Immunoregulatory effects and therapeutic potential of vitamin D in multiple sclerosis.维生素 D 在多发性硬化症中的免疫调节作用和治疗潜力。
Br J Pharmacol. 2020 Sep;177(18):4113-4133. doi: 10.1111/bph.15201. Epub 2020 Aug 5.
10
Bioactive Lipid Signaling in Cardiovascular Disease, Development, and Regeneration.生物活性脂质信号在心血管疾病、发育和再生中的作用。
Cells. 2020 Jun 3;9(6):1391. doi: 10.3390/cells9061391.
本文引用的文献
1
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
2
Chemical synthesis of [1 beta-3H] 1 alpha, 25-dihydroxyvitamin D3 and [1 alpha-3H] 1 beta, 25-dihydroxyvitamin D2: biological activity of 1 beta, 25-dihydroxyvitamin D3.[1β-³H]1α,25-二羟基维生素D3和[1α-³H]1β,25-二羟基维生素D2的化学合成：1β,25-二羟基维生素D3的生物活性
Biochem Biophys Res Commun. 1980 Dec 16;97(3):1031-7. doi: 10.1016/0006-291x(80)91479-5.
3
24,24-difluoro-1,25-dihydroxyvitamin D3: in vitro production, isolation, and biological activity.
Arch Biochem Biophys. 1980 Feb;199(2):473-8. doi: 10.1016/0003-9861(80)90304-5.
4
Inhibition of 25-hydroxyvitamin D3-1-hydroxylase by chronic metabolic acidosis.慢性代谢性酸中毒对25-羟基维生素D3-1-羟化酶的抑制作用。
Am J Physiol. 1982 Oct;243(4):E265-71. doi: 10.1152/ajpendo.1982.243.4.E265.
5
1,25-Dihydroxyvitamin D3 increases synthesis of the vitamin K-dependent bone protein by osteosarcoma cells.1,25-二羟基维生素D3可增加骨肉瘤细胞中维生素K依赖性骨蛋白的合成。
J Biol Chem. 1980 Dec 25;255(24):11660-3.
6
7
C(24)- and C(23)-oxidation, converging pathways of intestinal 1,25-dihydroxyvitamin D3 metabolism: identification of 24-keto-1,23,25-trihydroxyvitamin D3.
Biochemistry. 1983 Dec 6;22(25):5848-53. doi: 10.1021/bi00294a025.
8
The isolation and identification of two new metabolites of 25-hydroxyvitamin D3 produced in the kidney.
J Biol Chem. 1983 Nov 10;258(21):12920-8.
9
10
Calcium transport in perfused duodena from normal chicks: enhancement within fourteen minutes of exposure to 1,25-dihydroxyvitamin D3.正常雏鸡灌注十二指肠中的钙转运：暴露于1,25 - 二羟基维生素D3后十四分钟内增强。
Endocrinology. 1984 Oct;115(4):1476-83. doi: 10.1210/endo-115-4-1476.
Zotero 插件