Onoprienko L V, Mikhaleva I I, Lunev V E, Nesmeianov V A, Ivanov V T
Bioorg Khim. 1989 Jul;15(7):908-21.
Synthesis of peptides corresponding to the 59-72 and 25-36 sequences of human IL-2 is reported. The former peptide, which had been shown to be immunogenic in the protein molecule, was prepared to obtain antipeptide antibodies for isolation and purification of the recombinant IL-2. We located the epitope at the C-terminus of this peptide. In accordance with the IL-2 secondary structure and hydrophilicity profile analysis, the 25-36 fragment was chosen as the potential epitope. The peptides were synthesized by conventional methods in solution, conjugated with a protein carrier, and polyclonal rabbit antisera were obtained. Antibodies against both peptides were shown to be specific to human IL-2 in ELISA. Besides, monoclonal antibodies to IL-2 recognized in ELISA the 59-72 peptide, suggesting the epitope located in this region to be main one in the protein molecule.
报道了对应于人白细胞介素-2（IL-2）59 - 72和25 - 36序列的肽的合成。前一种肽已被证明在蛋白质分子中具有免疫原性，制备它是为了获得用于分离和纯化重组IL-2的抗肽抗体。我们将该肽的表位定位在其C末端。根据IL-2二级结构和亲水性图谱分析，选择25 - 36片段作为潜在表位。这些肽通过常规的溶液法合成，与蛋白质载体偶联，并获得了兔多克隆抗血清。在酶联免疫吸附测定（ELISA）中，针对这两种肽的抗体均显示对人IL-2具有特异性。此外，在ELISA中，IL-2单克隆抗体识别59 - 72肽，表明位于该区域的表位是蛋白质分子中的主要表位。
