Antonenko V V, Prudchenko I A, Mikhaleva I I, Mazurenko N N, Kiselev F L
Bioorg Khim. 1987 Oct;13(10):1301-12.
Synthesis of peptides corresponding to the 201-210 and 200-206 sequences of p28sis oncoprotein is reported. This region of the oncoprotein was chosen as a probable epitope basing on the analysis of its secondary structure and hydrophilicity profile. The synthesis was carried out by classical method in solution using side-protecting groups of the benzyl type. Conjugates of the peptides with protein carriers and polyclonal antibodies to the peptides were obtained. Antiserum against the 201-210 peptide conjugate is shown to be specific to p28sis oncoprotein and its p56sis dimer.
据报道，合成了与p28sis癌蛋白的201 - 210和200 - 206序列相对应的肽段。基于对该癌蛋白二级结构和亲水性的分析，选择该区域作为可能的表位。采用经典的溶液法，使用苄基型侧链保护基团进行合成。获得了肽段与蛋白质载体的缀合物以及针对这些肽段的多克隆抗体。结果显示，针对201 - 210肽段缀合物的抗血清对p28sis癌蛋白及其p56sis二聚体具有特异性。
