Kohli E, Maurice L, Bourgeois C, Bour J B, Pothier P
Laboratoire de Virologie, Facultés de Médecine et de Pharmacie, Dijon, France.
Virology. 1993 May;194(1):110-6. doi: 10.1006/viro.1993.1240.
Three hundred and ninety-one consecutive heptapeptides derived from the VP6 protein of bovine rotavirus (397 AA) were synthesized using the "pepscan" method and were assayed on the synthesis pins with monoclonal antibodies to VP6. Heptapeptides reactive with MAbs were located in four main regions: regions AA 32-64, AA 155-167, AA 208-274, and a fourth region at the C-terminal, from AA 380 to AA 397. Among these regions, two sequences were also reactive with the MAbs when longer peptides were assayed. The sequence located between AA 58 and AA 62 (NWNFD), recognized by MAbs RV-1026, RV-50, and RV-443, was previously reported. A new site was defined in the region essential for trimerization, between AA 159 and AA 165 (PYSASFT), which was recognized by MAbs RV-133 and RV-138.
采用“肽扫描”方法合成了391个源自牛轮状病毒VP6蛋白（397个氨基酸）的连续七肽，并在合成针上用抗VP6单克隆抗体进行检测。与单克隆抗体反应的七肽位于四个主要区域：氨基酸32 - 64区域、氨基酸155 - 167区域、氨基酸208 - 274区域以及C末端的第四个区域，从氨基酸380到氨基酸397。在这些区域中，当检测较长肽段时，有两个序列也与单克隆抗体反应。位于氨基酸58和氨基酸62之间（NWNFD）的序列，被单克隆抗体RV - 1026、RV - 50和RV - 443识别，此前已有报道。在三聚化关键区域（氨基酸159和氨基酸165之间，PYSASFT）定义了一个新位点，被单克隆抗体RV - 133和RV - 138识别。
